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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rat / IgG2a|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1:500|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:50-1:500|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-22646 detects CD34 in mouse and human samples. This antibody detects a neuraminidase sensitive epitope on endothelium in vivo, particullary on small vessels and neoformed capillaries and develpoing vascular structures in embryonal structures. Furthermore, the antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells.
MA1-22646 has been successfully used in ELISA, FACS, immunofluorescence, immunohistochemistry (frozen and paraffin), immunoprecipitation, and Western blot procedures.
The MA1-22646 immunogen is mouse CD34 protein.
Store at 4°C short term. For extended storage, aliqout and store at -20°C, avoiding freeze/thaw cycles.
CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD34 antigen; cluster designation 34; hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2