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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1:500|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
MA1-22646 detects CD34 in mouse and human samples. This antibody detects a neuraminidase sensitive epitope on endothelium in vivo, particullary on small vessels and neoformed capillaries and develpoing vascular structures in embryonal structures. Furthermore, the antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells.
MA1-22646 has been successfully used in ELISA, FACS, immunofluorescence, immunohistochemistry (frozen and paraffin), immunoprecipitation, and Western blot procedures.
The MA1-22646 immunogen is mouse CD34 protein.
Store at 4°C short term. For extended storage, aliqout and store at -20°C, avoiding freeze/thaw cycles.
CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Myeloproliferative defects following targeting of the Drf1 gene encoding the mammalian diaphanous related formin mDia1.
MA1-22646 was used in flow cytometry to indicate that defective DRF1 expression or mammalian diaphanous 1 function contributes to myeloid malignancies and suggest mammalian diaphanous 1 as an attractive therapeutic target in human myeloproliferative synd
|Peng J,Kitchen SM,West RA,Sigler R,Eisenmann KM,Alberts AS||Cancer research (67:7565)||2007|
CD34; CD34 antigen; cluster designation 34; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2