Invitrogen

CD34 Monoclonal Antibody (1H6), PE, eBioscience™

Product Image Gallery

CD34 Antibody in Flow Cytometry (Flow) — Staining of a canine CD34-expressing cell line with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Canine CD34 PE (purple histogram). Total viable cells were used for analysis.

Product Details

12-0340-42

Applications	Tested Dilution	Publications
Immunohistochemistry (PFA fixed) (IHC (PFA))	-	View 1 publication 1 publication
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	View 4 publications 4 publications

Product Specifications
Species Reactivity	Dog
Published species	Dog
Host/Isotype	Mouse / IgG1
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	1H6
Conjugate	PE
Excitation/Emission Max	565/576 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10668490

Product Specific Information

Description: The 1H6 monoclonal antibody reacts with canine CD34, also known as mucosialin. CD34 belongs to a protein family that also includes endoglycan and podocalyxin. These single-pass transmembrane proteins possess heavily glycosylated extracellular and N-terminal mucin domains. Similar to humans, canine CD34 can be detected on hematopoietic progenitor cells in the bone marrow, peripheral blood and hair follicle, as well as on some populations of mesenchymal stem cells, tumor cell lines, and on vascular endothelia in the adult. CD34 can be used as a marker to isolate cells capable of hematopoietic cell engraftment.

The 1H6 antibody has been reported to work for enriching CD34+ cells.

Applications Reported: This 1H6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1H6 antibody has been pre-titrated and tested by flow cytometric analysis of a canine CD34-expressing cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells.

Stem cells and cloning : advances and applications

de Oliveira Pinheiro A,Lara VM,Souza AF,Casals JB,Bressan FF,Fantinato Neto P,Oliveira VC,Martins DS,Ambrosio CE

12-0340 was used in Flow cytometry/Cell sorting to show that the gestational stage is a key factor that may influence the functionality of therapies when using fetal membrane tissues from different periods of pregnancy.

Thu Apr 14 00:00:00 EDT 2022

Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells.

Stem cells and cloning : advances and applications

de Oliveira Pinheiro A,Lara VM,Souza AF,Casals JB,Bressan FF,Fantinato Neto P,Oliveira VC,Martins DS,Ambrosio CE

Thu Apr 14 00:00:00 EDT 2022

Comparison of the Efficacy of Surgical Decompression Alone and Combined With Canine Adipose Tissue-Derived Stem Cell Transplantation in Dogs With Acute Thoracolumbar Disk Disease and Spinal Cord Injury.

Frontiers in veterinary science

Bach FS,Rebelatto CLK,Fracaro L,Senegaglia AC,Fragoso FYI,Daga DR,Brofman PRS,Pimpão CT,Engracia Filho JR,Montiani-Ferreira F,Villanova JA

12-0340 was used in Flow cytometry/Cell sorting to compare the effects of surgical decompression alone and combined with an allogeneic transplantation of canine adipose tissue-derived mesenchymal stem cells in the treatment of dogs with acute paraplegia.

Thu Oct 01 00:00:00 EDT 2020

Adenovirus-Mediated Expression of BMP-2 and BFGF in Bone Marrow Mesenchymal Stem Cells Combined with Demineralized Bone Matrix For Repair of Femoral Head Osteonecrosis in Beagle Dogs.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Peng WX,Wang L

12-0340 was used in Flow cytometry/Cell sorting to indicate that the use of Ad-BMP-2/bFGF-modified BMSCs in conjunction with DBM could successfully repair ONFH in a dog model by promoting bone formation and angiogenesis.

Mon Dec 18 00:00:00 EST 2017

The canine epiphyseal-derived mesenchymal stem cells are comparable to bone marrow derived-mesenchymal stem cells.

The Journal of veterinary medical science

Chang YP,Hong HP,Lee YH,Liu IH

12-0340 was used in Flow cytometry/Cell sorting to develop a novel method to isolate mesenchymal stem cells from an alternative canine source.

Sun Mar 01 00:00:00 EST 2015

Corrective GUSB transfer to the canine mucopolysaccharidosis VII cornea using a helper-dependent canine adenovirus vector.

Journal of controlled release : official journal of the Controlled Release Society

Serratrice N,Cubizolle A,Ibanes S,Mestre-Francés N,Bayo-Puxan N,Creyssels S,Gennetier A,Bernex F,Verdier JM,Haskins ME,Couderc G,Malecaze F,Kalatzis V,Kremer EJ

12-0340 was used in Immunofluorescence to test the efficacy of canine adenovirus type 2 vectors to transduce keratocyte in vivo and ex vivo.

Sat May 10 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell marker CD34; Mucosialin; RP11-328D5.2; sialomucin protein; type 1 transmembrane protein

Gene Aliases: CD34

UniProt ID: (Dog) Q28270

Entrez Gene ID: (Dog) 415130

Molecular Function: cell adhesion molecule

Function(s)

tissue homeostasis glomerular filtration regulation of blood pressure negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis leukocyte migration positive regulation of transforming growth factor beta production cell-cell adhesion negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of neuron death positive regulation of vasculogenesis

Process(es)

carbohydrate binding

CD34 Monoclonal Antibody (1H6), PE, eBioscience™

Product Details

12-0340-42

Immunohistochemistry (PFA fixed) (IHC (PFA))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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