|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human peripheral blood monocytes from rheumatoid arthritis patient and human cell line KG-1a|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 µl/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is a monoclonal mixture of FITC-conjugated CD45 (clone BHPT-1, isotype IgG1) and PE-conjugated CD34 (clone CS37, isotype IgG1).
Identification of all human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinophils and basophils in peripheral blood expressing the 180-220 k D M.W. T200 family surface antigen. CD45 is also present on thymus, spleen, tonsil and progenitor cells in bone marrow. Human Progenitor Cell Antigen (MW 115 K daltons) is found on normal peripheral blood lymphocytes, monocytes, granulocytes and platelets. CD34 is present on 1% or less of cells in normal human bone marow. Human Progenitor Cell Antige reacts with 30-70 % of acute leukemias, as well as all TdT positive lymphocytes in bone marrow.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.