Immunofluorescent analysis of CD35 (green) showing staining in the cytoplasm and membrane of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD35 monoclonal antibody (Product # MA5-13122) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Intact human monocytes|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-13122 targets CD35 in FACS, ICC/IF, and IHC (P) applications and shows reactivity with Human and Non-human primate samples.
The MA5-13122 immunogen is intact human monocytes.
Complement receptor 1 (CR1, CD35) is a cell membrane-bound, monomeric glycoprotein that is found on numerous cell types of primates including erythrocytes, lymphocytes, and phagocytes The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effect of B-cell depletion on coreceptor switching in R5 simian-human immunodeficiency virus infection of rhesus macaques.
MA5-13122 was used in immunohistochemistry to study the effect of B-cell depletion on co-receptor switching in rhesus macaques infected with R5 simian-human immunodeficiency virus.
|Tasca S,Zhuang K,Gettie A,Knight H,Blanchard J,Westmoreland S,Cheng-Mayer C||Journal of virology (85:3086)||2011|