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Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human spleen (A) and human tonsil (B) sections at 400x magnification with DAPI counterstain. Primary antibody used was a CD35/CR1 monoclonal antibody (clone To5) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-mouse Ig at a 1:500 dilution and Normal Goat Serum (blocking)
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Mouse monoclonal antibodies raised against human C3b receptor-enriched precipitates from tonsil cell membranes.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17711 targets CD35/CR1 in FACS, IHC (F), and WB applications and shows reactivity with Human samples.
The MA5-17711 immunogen is mouse monoclonal antibodies raised against human C3b receptor-enriched precipitates from tonsil cell membranes.
This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C3-binding protein; C3b/C4b receptor; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); complement component receptor 1; complement receptor type 1; Knops blood group antigen
C3BR; C4BR; CD35; CR1; KN