Immunofluorescent analysis of CD36 (green) showing staining in the membrane and cytoplasm of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD36 monoclonal antibody (Product # MA5-14112) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Stimulated human leukocytes|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1 ug/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14112 targets CD36 in FACS, immunofluorescence, and immunohistochemistry (frozen) applications and shows reactivity with human and mouse samples.
The MA5-14112 immunogen is stimulated human leukocytes.
CD36 (also known as platelet glycoprotein IV, OKM5-antigen, or platelet glycoprotein IIIb) acts as a receptor for thrombospondin (TSP), collagen, falciparum malaria parasitized erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL), and apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischaemia observed in sickle cell disease and cerebral malaria. Ab-3 blocks adhesion of P. falciparum parasitized red blood cells to CD36 and strongly inhibits collagen-induced platelet aggregation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma.
MA5-14112 was used in flow cytometry to evaluate the tumor-associated antigen alpha-fetoprotein as a target for immunotherapy
|Pardee AD,Yano H,Weinstein AM,Ponce AA,Ethridge AD,Normolle DP,Vujanovic L,Mizejewski GJ,Watkins SC,Butterfield LH||Journal for immunotherapy of cancer (3:null)||2015|
Implication of scavenger receptors in the interactions between diesel exhaust particles and immature or mature dendritic cells.
MA5-14112 was used in flow cytometry to study the involvement of scavenger receptors in toxicopathology of diesel exhaust particles
|Taront S,Dieudonné A,Blanchard S,Jeannin P,Lassalle P,Delneste Y,Gosset P||Particle and fibre toxicology (6:null)||2009|
The cysteine-rich interdomain region from the highly variable plasmodium falciparum erythrocyte membrane protein-1 exhibits a conserved structure.
MA5-14112 was used in flow cytometry to study the conserved structure of the cysteine-rich interdomain region from the highly variable Plasmodium falciparum erythrocyte membrane protein-1
|Klein MM,Gittis AG,Su HP,Makobongo MO,Moore JM,Singh S,Miller LH,Garboczi DN||PLoS pathogens (4:null)||2008|
Histopathologies, immunolocalization, and a glycan binding screen provide insights into Plasmodium falciparum interactions with the human placenta.
MA5-14112 was used in immunohistochemistry to study the mechanisms underlying the interaction of Plasmodium falciparum with the human placenta
|Hromatka BS,Ngeleza S,Adibi JJ,Niles RK,Tshefu AK,Fisher SJ||Biology of reproduction (88:null)||2013|
Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis.
MA5-14112 was used in immunohistochemistry To study the molecular mechanism for the arrest of metastatic tumour cells in human lymph node sinuses
|Martens JH,Kzhyshkowska J,Falkowski-Hansen M,Schledzewski K,Gratchev A,Mansmann U,Schmuttermaier C,Dippel E,Koenen W,Riedel F,Sankala M,Tryggvason K,Kobzik L,Moldenhauer G,Arnold B,Goerdt S||The Journal of pathology (208:574)||2006|
Inhibition of endothelial cell migration by thrombospondin-1 type-1 repeats is mediated by beta1 integrins.
MA5-14112 was used in blocking or activating experiment to investigate the mechanism for anti-angiogenic effect of thrombospondin-1
|Short SM,Derrien A,Narsimhan RP,Lawler J,Ingber DE,Zetter BR||The Journal of cell biology (168:643)||2005|
Phosphatidylserine regulates the maturation of human dendritic cells.
MA5-14112 was used in blocking or activating experiment to study the role of phosphatidylserine in regulating human dendritic cell maturation
|Chen X,Doffek K,Sugg SL,Shilyansky J||Journal of immunology (Baltimore, Md. : 1950) (173:2985)||2004|