Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of CD36 (green) showing staining in the membrane and cytoplasm of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD36 monoclonal antibody (Product # MA5-14112) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Stimulated human leukocytes|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1 ug/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14112 targets CD36 in FACS, immunofluorescence, and immunohistochemistry (frozen) applications and shows reactivity with human and mouse samples.
The MA5-14112 immunogen is stimulated human leukocytes.
CD36 (also known as platelet glycoprotein IV, OKM5-antigen, or platelet glycoprotein IIIb) acts as a receptor for thrombospondin (TSP), collagen, falciparum malaria parasitized erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL), and apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischaemia observed in sickle cell disease and cerebral malaria. Ab-3 blocks adhesion of P. falciparum parasitized red blood cells to CD36 and strongly inhibits collagen-induced platelet aggregation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma.
MA5-14112 was used in flow cytometry to evaluate the tumor-associated antigen alpha-fetoprotein as a target for immunotherapy
|Pardee AD,Yano H,Weinstein AM,Ponce AA,Ethridge AD,Normolle DP,Vujanovic L,Mizejewski GJ,Watkins SC,Butterfield LH||Journal for immunotherapy of cancer (3:null)||2015|
Implication of scavenger receptors in the interactions between diesel exhaust particles and immature or mature dendritic cells.
MA5-14112 was used in flow cytometry to study the involvement of scavenger receptors in toxicopathology of diesel exhaust particles
|Taront S,Dieudonné A,Blanchard S,Jeannin P,Lassalle P,Delneste Y,Gosset P||Particle and fibre toxicology (6:null)||2009|
The cysteine-rich interdomain region from the highly variable plasmodium falciparum erythrocyte membrane protein-1 exhibits a conserved structure.
MA5-14112 was used in flow cytometry to study the conserved structure of the cysteine-rich interdomain region from the highly variable Plasmodium falciparum erythrocyte membrane protein-1
|Klein MM,Gittis AG,Su HP,Makobongo MO,Moore JM,Singh S,Miller LH,Garboczi DN||PLoS pathogens (4:null)||2008|
Histopathologies, immunolocalization, and a glycan binding screen provide insights into Plasmodium falciparum interactions with the human placenta.
MA5-14112 was used in immunohistochemistry to study the mechanisms underlying the interaction of Plasmodium falciparum with the human placenta
|Hromatka BS,Ngeleza S,Adibi JJ,Niles RK,Tshefu AK,Fisher SJ||Biology of reproduction (88:null)||2013|
Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis.
MA5-14112 was used in immunohistochemistry To study the molecular mechanism for the arrest of metastatic tumour cells in human lymph node sinuses
|Martens JH,Kzhyshkowska J,Falkowski-Hansen M,Schledzewski K,Gratchev A,Mansmann U,Schmuttermaier C,Dippel E,Koenen W,Riedel F,Sankala M,Tryggvason K,Kobzik L,Moldenhauer G,Arnold B,Goerdt S||The Journal of pathology (208:574)||2006|
Inhibition of endothelial cell migration by thrombospondin-1 type-1 repeats is mediated by beta1 integrins.
MA5-14112 was used in blocking or activating experiment to investigate the mechanism for anti-angiogenic effect of thrombospondin-1
|Short SM,Derrien A,Narsimhan RP,Lawler J,Ingber DE,Zetter BR||The Journal of cell biology (168:643)||2005|
Phosphatidylserine regulates the maturation of human dendritic cells.
MA5-14112 was used in blocking or activating experiment to study the role of phosphatidylserine in regulating human dendritic cell maturation
|Chen X,Doffek K,Sugg SL,Shilyansky J||Journal of immunology (Baltimore, Md. : 1950) (173:2985)||2004|
CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); cluster determinant 36; FAT; fatty acid translocase; glycoprotein IIIb; GPIIIB; GPIV; leukocyte differentiation antigen CD36; PAS IV; PAS-4; PAS-4 protein; platelet glycoprotein 4; platelet glycoprotein IV; Scarb3; scavenger receptor class B, member 3
BDPLT10; CD36; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3