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Invitrogen
CD36 Monoclonal Antibody (HM36), Super Bright™ 600, eBioscience™
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Product Details
63-0362-82
| Applications | Tested Dilution | Publications |
|---|---|---|
Flow Cytometry (Flow) |
1 µg/test | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Mouse |
Published species |
Not Applicable |
Host/Isotype |
Armenian hamster / IgG |
Recommended Isotype Control |
Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 600, eBioscience™ |
Class |
Monoclonal |
Type |
Antibody |
Clone |
HM36 |
Immunogen |
CHO cells transfected with full-length mouse CD36 |
Conjugate |
Super Bright™ 600
|
Excitation/Emission Max |
414/601 nm
View spectra
|
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2, with BSA |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2734971 |
Product Specific Information
Description: This HM36 antibody recognizes mouse CD36, also known as scavenger receptor BIII.
Applications Reported: This HM36 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HM36 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD36 (fatty acid translocase, FAT) is an 88 kDa, ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similar to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. Further, CD36 may function as a cell adhesion molecule and directly mediates cyto-adherence of Plasmodium falciparum parasitized erythrocytes. Mutations in the CD36 gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the CD36 protein have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Flow Cytometry
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Bioinformatics
Protein Aliases: CD36; fatty acid translocase; Glycoprotein IIIb; GPIIIB; GPIV; PAS IV; PAS-4; Platelet glycoprotein 4; Platelet glycoprotein IV; SR-B3
Gene Aliases: Cd36; FAT; GPIV; Scarb3
UniProt ID: (Mouse) Q08857
Entrez Gene ID: (Mouse) 12491
Molecular Function:
membrane trafficking regulatory protein
negative regulation of transcription from RNA polymerase II promoter
long-chain fatty acid metabolic process
positive regulation of cell-matrix adhesion
pattern recognition receptor signaling pathway
negative regulation of systemic arterial blood pressure
fatty acid metabolic process
triglyceride metabolic process
transport
receptor-mediated endocytosis
phagocytosis, recognition
cell adhesion
signal transduction
cell surface receptor signaling pathway
nitric oxide mediated signal transduction
response to mechanical stimulus
negative regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol storage
fatty acid transport
long-chain fatty acid transport
plasma membrane long-chain fatty acid transport
negative regulation of angiogenesis
fatty acid oxidation
lipid storage
cGMP-mediated signaling
positive regulation of blood coagulation
cholesterol transport
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
plasma lipoprotein particle clearance
low-density lipoprotein particle clearance
lipoprotein transport
negative regulation of transcription factor import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
apoptotic cell clearance
positive regulation of MAPK cascade
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
negative regulation of growth of symbiont in host
long-chain fatty acid import
positive regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-positive bacterium
low-density lipoprotein particle mediated signaling
positive regulation of phagocytosis, engulfment
positive regulation of macrophage cytokine production
cellular response to bacterial lipopeptide
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to hydroperoxide
regulation of removal of superoxide radicals
positive regulation of blood microparticle formation
positive regulation of reactive oxygen species metabolic process
low-density lipoprotein receptor activity
protein binding
high-density lipoprotein particle binding
lipid binding
low-density lipoprotein particle binding
transforming growth factor beta binding
thrombospondin receptor activity
oleic acid binding
lipoteichoic acid receptor activity
lipoprotein particle binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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