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Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Flow Cytometry (Flow) |
1 µg/test | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Mouse |
Published species |
Not Applicable |
Host/Isotype |
Armenian hamster / IgG |
Recommended Isotype Control |
Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 600, eBioscience™ |
Class |
Monoclonal |
Type |
Antibody |
Clone |
HM36 |
Immunogen |
CHO cells transfected with full-length mouse CD36 |
Conjugate |
Super Bright™ 600
|
Excitation/Emission Max |
414/601 nm View spectra |
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2, with BSA |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2734971 |
Description: This HM36 antibody recognizes mouse CD36, also known as scavenger receptor BIII.
Applications Reported: This HM36 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HM36 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
CD36 (fatty acid translocase, FAT) is an 88 kDa, ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similar to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. Further, CD36 may function as a cell adhesion molecule and directly mediates cyto-adherence of Plasmodium falciparum parasitized erythrocytes. Mutations in the CD36 gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the CD36 protein have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Protein Aliases: CD36; fatty acid translocase; Glycoprotein IIIb; GPIIIB; GPIV; PAS IV; PAS-4; Platelet glycoprotein 4; Platelet glycoprotein IV; SR-B3
Gene Aliases: Cd36; FAT; GPIV; Scarb3
UniProt ID: (Mouse) Q08857
Entrez Gene ID: (Mouse) 12491
Molecular Function:
membrane trafficking regulatory protein
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