Flow cytometry analysis of CD37 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA5-13567) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Spleen cells of a patient with hairy cell leukemia|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13567 targets CD37 in FACS, IF, and IHC (F) applications and shows reactivity with Human samples.
The MA5-13567 immunogen is spleen cells of a patient with hairy cell leukemia.
CD37, a tetraspan transmembrane protein, is strongly expressed on normal and neoplastic mature (sIg+) B lymphocytes. In B-cell ontogeny, CD37 appears after the pre-B-cell stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD37 is absent from platelets, erythrocytes. It associates with other tetraspan transmembrane proteins and MHC class II molecules to form a large complexes at the surface of B cells and play a role in signal transduction. CD37 is a valuable and stable marker for peripheral mature B-cells and corresponding malignancies like B-cell chronic lymphocytic leukemia (B-CLL), hairy cell leukemia (HCL), and all types of B-cell non-Hodgkin lymphoma (B-NHL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.