Invitrogen

CD38 Monoclonal Antibody (HIT2), Alexa Fluor™ 700, eBioscience™

Product Image Gallery

CD38 Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Alexa Fluor® 700 (Product # 56-4714-80) (open histogram) or Anti-Human CD38 Alexa Fluor® 700 (filled histogram). Cells in the lymphocyte (left) and monocyte (right) gates were used for analysis.

CD38 Antibody in Immunocytochemistry (ICC/IF) — Fig. 1 Marker verification and validation of independent markers in BALL cells by immunofluorescence and flow cytometry. The expression of CD20 ( a - c ) and CD38 ( d - f ), were positive on BALL cell membranes. CD90f ( g - i ) and CD49f ( j , k ) were not expressed in BALL cells. DAPI indicates the cell nucleus. This finding was confirmed via the conducted flow cytometry analysis. Scale bar = 100 mum

Product Details

56-0389-42

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	5 µL (0.5 µg)/test	View 16 publications 16 publications

Product Specifications
Species Reactivity	Human
Published species	Chimpanzee, Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	HIT2
Conjugate	Alexa Fluor™ 700 APC APC-eFluor 780 Biotin Brilliant Violet 421 eFluor 450 FITC PE PE-Cyanine5 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 702 Unconjugated
Excitation/Emission Max	696/719 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10804040

Product Specific Information

Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.

Applications Reported: This HIT2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.

Frontiers in immunology

Lecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A

56-0389 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment.

Thu Mar 31 00:00:00 EDT 2022

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD38 monoclonal antibody (Product # 56-0389-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

Published figure using CD38 monoclonal antibody (Product # 56-0389-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen dysregulates expression of MCL-1 by targeting FBW7.

PLoS pathogens

Kim YJ,Kim Y,Kumar A,Kim CW,Toth Z,Cho NH,Lee HR

Published figure using CD38 monoclonal antibody (Product # 56-0389-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Intrathecal B Cells in MS Have Significantly Greater Lymphangiogenic Potential Compared to B Cells Derived From Non-MS Subjects.

Frontiers in neurology

Stein J,Xu Q,Jackson KC,Romm E,Wuest SC,Kosa P,Wu T,Bielekova B

56-0389 was used in Flow cytometry/Cell sorting to compare the activity of intrathecal B cells in MS and non-MS subjects, revealing a higher lymphangiogenic potential in B cells taken from MS subjects.

Wed Sep 30 00:00:00 EDT 2020

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

Published figure using CD38 monoclonal antibody (Product # 56-0389-42) in Immunocytochemistry

Mon Dec 16 00:00:00 EST 2019

IFNγ induces epigenetic programming of human T-bet^hi B cells and promotes TLR7/8 and IL-21 induced differentiation.

eLife

Zumaquero E,Stone SL,Scharer CD,Jenks SA,Nellore A,Mousseau B,Rosal-Vela A,Botta D,Bradley JE,Wojciechowski W,Ptacek T,Danila MI,Edberg JC,Bridges SL,Kimberly RP,Chatham WW,Schoeb TR,Rosenberg AF,Boss JM,Sanz I,Lund FE

Published figure using CD38 monoclonal antibody (Product # 56-0389-42) in Flow Cytometry

Wed May 15 00:00:00 EDT 2019

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD38 monoclonal antibody (Product # 56-0389-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways.

Genome biology

Psaila B,Barkas N,Iskander D,Roy A,Anderson S,Ashley N,Caputo VS,Lichtenberg J,Loaiza S,Bodine DM,Karadimitris A,Mead AJ,Roberts I

56-0389 was used in Flow cytometry/Cell sorting to clarify the cellular hierarchy in human megakaryocyte/erythroid lineage commitment and highlight the importance of using a combination of single-cell approaches to dissect cellular heterogeneity.

Tue May 03 00:00:00 EDT 2016

Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Dabas R,Lee R,Servito MT,Dharmani-Khan P,Modi M,van Slyke T,Luider J,Durand C,Larratt L,Brandwein J,Morris D,Daly A,Khan FM,Storek J

Sun May 01 00:00:00 EDT 2016

A novel autosomal recessive TERT T1129P mutation in a dyskeratosis congenita family leads to cellular senescence and loss of CD34+ hematopoietic stem cells not reversible by mTOR-inhibition.

Aging

Stockklausner C,Raffel S,Klermund J,Bandapalli OR,Beier F,Brümmendorf TH,Bürger F,Sauer SW,Hoffmann GF,Lorenz H,Tagliaferri L,Nowak D,Hofmann WK,Buergermeister R,Kerber C,Rausch T,Korbel JO,Luke B,Trumpp A,Kulozik AE

56-0389 was used in Flow cytometry/Cell sorting to outline limitations of a potential dyskeratosis congenita therapy based on rapamycin.

Sun Nov 01 00:00:00 EDT 2015

Inhibitory Receptor Expression Depends More Dominantly on Differentiation and Activation than "Exhaustion" of Human CD8 T Cells.

Frontiers in immunology

Legat A,Speiser DE,Pircher H,Zehn D,Fuertes Marraco SA

56-0389 was used in Flow cytometry/Cell sorting to evaluate the direct relationship between inhibitory receptor expression and functionality in CD8 T cells.

Mon Jan 06 00:00:00 EST 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 56-0389-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 56-0389-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Hypermutation of the inactive X chromosome is a frequent event in cancer.

Cell

Jäger N,Schlesner M,Jones DT,Raffel S,Mallm JP,Junge KM,Weichenhan D,Bauer T,Ishaque N,Kool M,Northcott PA,Korshunov A,Drews RM,Koster J,Versteeg R,Richter J,Hummel M,Mack SC,Taylor MD,Witt H,Swartman B,Schulte-Bockholt D,Sultan M,Yaspo ML,Lehrach H,Hutter B,Brors B,Wolf S,Plass C,Siebert R,Trumpp A,Rippe K,Lehmann I,Lichter P,Pfister SM,Eils R

56-0389 was used in Flow cytometry/Cell sorting to suggest that hypermutation of the inactive X chromosome is an early and frequent feature of tumorigenesis resulting from DNA replication stress.

Thu Oct 24 00:00:00 EDT 2013

Development of Norwalk virus-specific monoclonal antibodies with therapeutic potential for the treatment of Norwalk virus gastroenteritis.

Journal of virology

Chen Z,Sosnovtsev SV,Bok K,Parra GI,Makiya M,Agulto L,Green KY,Purcell RH

56-0389 was used in Flow cytometry/Cell sorting to show that norovirus-neutralizing monoclonal antibodies can block carbohydrate binding and inhibit hemagglutination by Norwalk virus recombinant virus-like particles.

Sun Sep 01 00:00:00 EDT 2013

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD38 monoclonal antibody (Product # 56-0389-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Multiparametric flow cytometry for identification and fluorescence activated cell sorting of five distinct B-cell subpopulations in normal tonsil tissue.

American journal of clinical pathology

Kjeldsen MK,Perez-Andres M,Schmitz A,Johansen P,Boegsted M,Nyegaard M,Gaihede M,Bukh A,Johnsen HE,Orfao A,Dybkaer K

56-0389 was used in Flow cytometry/Cell sorting to establish a procedure capable of isolating distinct B-cell subpopulations from human tonsils as a basis for subsequent molecular analyses.

Thu Dec 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPr hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; NAD(+) nucleosidase; NAD+ nucleosidase; T10

Gene Aliases: ADPRC 1; ADPRC1; CD38

UniProt ID: (Human) P28907

Entrez Gene ID: (Human) 952

Molecular Function: cyclase lyase metabolite interconversion enzyme

Function(s)

response to hypoxia signal transduction positive regulation of cytosolic calcium ion concentration female pregnancy positive regulation of cell growth positive regulation of B cell proliferation positive regulation of insulin secretion response to estradiol response to retinoic acid response to progesterone response to hydroperoxide response to drug negative regulation of apoptotic process negative regulation of bone resorption negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of vasoconstriction B cell receptor signaling pathway long term synaptic depression response to interleukin-1 apoptotic signaling pathway

Process(es)

NAD+ nucleosidase activity transferase activity phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity

CD38 Monoclonal Antibody (HIT2), Alexa Fluor™ 700, eBioscience™

Product Details

56-0389-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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