|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human CD3 delta|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 5 publications below|
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.
Class 2 Device
Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.
MHCD0301-4 was used in flow cytometry to assess the effects of a TransFix-based stabilization technique on leukocyte scatter characteristics, immunophenotyping, membrane permeability, absolute cell counting and morphology.
|Canonico B,Zamai L,Burattini S,Granger V,Mannello F,Gobbi P,Felici C,Falcieri E,Reilly JT,Barnett D,Papa S||Journal of immunological methods (295:67)||2004|
Flow cytometry in the bone marrow staging of mature B-cell neoplasms.
MHCD0301-4 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.
|Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G||Cytometry. Part B, Clinical cytometry (54:10)||2003|
Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a. Interaction between monoclonal antibody clones, fluorochromes and sample preparation protocols.
MHCD0301-4 was used in flow cytometry to optimize B- and T-lymphoid lineage detection in acute leukemias by intracellular staining for myeloperoxidase, CD79a, and CD3.
|Kappelmayer J,Gratama JW,Karászi E,Menéndez P,Ciudad J,Rivas R,Orfao A||Journal of immunological methods (242:53)||2000|
Reactive oxygen intermediates during programmed cell death induced in the thymus of the Ts(1716)65Dn mouse, a murine model for human Down's syndrome.
MHCD0301-4 was used in flow cytometry to elucidate role of SOD-1 overexpression in thymocyte biology.
|Paz-Miguel JE,Flores R,Sánchez-Velasco P,Ocejo-Vinyals G,Escribano de Diego J,López de Rego J,Leyva-Cobián F||Journal of immunology (Baltimore, Md. : 1950) (163:5399)||1999|
T cell priming enhances IL-4 gene expression by increasing nuclear factor of activated T cells.
MHCD0301-4 was used in flow cytometry to study selective expression of cytokines by T cells.
|Cron RQ,Bort SJ,Wang Y,Brunvand MW,Lewis DB||Journal of immunology (Baltimore, Md. : 1950) (162:860)||1999|