Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452) and 0.5 µg of Armenian Hamster IgG Isotype Control PE-Cyanine7 (Product # 25-4888) (left) or 0.5 µg of Anti-Mouse CD3e PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.
Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma.||Geng H,Zhang GM,Xiao H,Yuan Y,Li D,Zhang H,Qiu H,He YF,Feng ZH||International journal of cancer (118:2657)||2006|
|Not Applicable||Not Cited||CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response.||Takeuchi A,Usui Y,Takeuchi M,Hattori T,Kezuka T,Suzuki J,Okunuki Y,Iwasaki T,Haino M,Matsushima K,Usui M||Investigative ophthalmology and visual science (46:3753)||2005|
||Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells.||Krieg C,Han P,Stone R,Goularte OD,Kaye J||Journal of immunology (Baltimore, Md. : 1950) (175:6420)||2005|
|Not Applicable||Not Cited||Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells.||Krieg C,Han P,Stone R,Goularte OD,Kaye J||Journal of immunology (Baltimore, Md. : 1950) (175:6420)||2005|