|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers.
Q10484 was used in flow cytometry to report the phase I trial results of CDX-301
|Anandasabapathy N,Breton G,Hurley A,Caskey M,Trumpfheller C,Sarma P,Pring J,Pack M,Buckley N,Matei I,Lyden D,Green J,Hawthorne T,Marsh HC,Yellin M,Davis T,Keler T,Schlesinger SJ||Bone marrow transplantation (50:924)||2015|
Simultaneous zinc-finger nuclease editing of the HIV coreceptors ccr5 and cxcr4 protects CD4+ T cells from HIV-1 infection.
Q10484 was used in flow cytometry to use zinc-finger nucleases to simultaneous modify ccr5 and cxcr4 in primary human CD4(+) T cells and introduce these cells into a humanized mouse model of HIV-1 infection.
|Didigu CA,Wilen CB,Wang J,Duong J,Secreto AJ,Danet-Desnoyers GA,Riley JL,Gregory PD,June CH,Holmes MC,Doms RW||Blood (123:61)||2014|
CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.
Q10484 was used in flow cytometry to analyze the proliferation and functional profile of circulating double positive T cells from acutely HIV-infected individuals and chronically HIV-infected viral controllers.
|Frahm MA,Picking RA,Kuruc JD,McGee KS,Gay CL,Eron JJ,Hicks CB,Tomaras GD,Ferrari G||Journal of immunology (Baltimore, Md. : 1950) (188:4289)||2012|
IL-7 and IL-21 are superior to IL-2 and IL-15 in promoting human T cell-mediated rejection of systemic lymphoma in immunodeficient mice.
Q10484 was used in flow cytometry to compare the therapeutic efficacy of CD19-specific human primary T cells that constitutively express gamma(c)-cytokines
|Markley JC,Sadelain M||Blood (115:3508)||2010|
|Not Applicable||Not Cited||
Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo.
Q10484 was used in flow cytometry to study facilitation of HIV-1 transmission to cervico-vaginal tissue ex vivo by interleukin-7
|Introini A,Vanpouille C,Lisco A,Grivel JC,Margolis L||PLoS pathogens (9:null)||2013|