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|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||H-2Kb - specific murine cytotoxic T-lymphocyte clone BM10-37.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25-1:200|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 2 publications below|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.
MA1-84019 was used in flow cytometry to determine the expression of CD39 in mouse and human T cells
|Borsellino G,Kleinewietfeld M,Di Mitri D,Sternjak A,Diamantini A,Giometto R,Höpner S,Centonze D,Bernardi G,Dell'Acqua ML,Rossini PM,Battistini L,Rötzschke O,Falk K||Blood (110:1225)||2007|
Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.
MA1-84019 was used in flow cytometry to investigate the changes of cytokine expression in NK cells after interacting with tumor cells
|Das S,Varalakshmi C,Kumari AL,Patel M,Khar A||Cancer immunology, immunotherapy : CII (50:428)||2001|
CD3-epsilon; CD3e antigen; epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; T-cell antigen receptor complex; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE
AI504783; CD3; Cd3e; CD3epsilon; T3e