|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||H-2Kb - specific murine cytotoxic T-lymphocyte clone BM10-37.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25-1:200|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Diet-Derived Short Chain Fatty Acids Stimulate Intestinal Epithelial Cells To Induce Mucosal Tolerogenic Dendritic Cells.
MA1-84019 was used in flow cytometry to assess the effects of dietary fiber and short chain fatty acids on the intestinal immune system and microbiota
|Goverse G,Molenaar R,Macia L,Tan J,Erkelens MN,Konijn T,Knippenberg M,Cook EC,Hanekamp D,Veldhoen M,Hartog A,Roeselers G,Mackay CR,Mebius RE||Journal of immunology (Baltimore, Md. : 1950) (198:2172)||2017|
DNAM-1 controls NK cell activation via an ITT-like motif.
MA1-84019 was used in flow cytometry to study how DNAM-1 controls NK cell-mediated cytotoxicity and cytokine production
|Zhang Z,Wu N,Lu Y,Davidson D,Colonna M,Veillette A||The Journal of experimental medicine (212:2165)||2015|
Innate immunological function of TH2 cells in vivo.
MA1-84019 was used in flow cytometry to report a role for effector T helper type 2 cells during T cell receptor-independent innate-like immune responses
|Guo L,Huang Y,Chen X,Hu-Li J,Urban JF,Paul WE||Nature immunology (16:1051)||2015|
Spatiotemporal requirements for IRF7 in mediating type I IFN-dependent susceptibility to blood-stage Plasmodium infection.
MA1-84019 was used in flow cytometry to determine the role of interferon regulatory factor 7 in Plasmodium berghei ANKA infection
|Edwards CL,Best SE,Gun SY,Claser C,James KR,de Oca MM,Sebina I,Rivera Fde L,Amante FH,Hertzog PJ,Engwerda CR,Renia L,Haque A||European journal of immunology (45:130)||2015|
|Not Applicable||Not Cited||
Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge.
MA1-84019 was used in flow cytometry to elucidate mechanisms that regulate antimicrobial protein production by Paneth cells
|Walker CR,Hautefort I,Dalton JE,Overweg K,Egan CE,Bongaerts RJ,Newton DJ,Cruickshank SM,Andrew EM,Carding SR||PloS one (8:null)||2013|
Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.
MA1-84019 was used in flow cytometry to determine the expression of CD39 in mouse and human T cells
|Borsellino G,Kleinewietfeld M,Di Mitri D,Sternjak A,Diamantini A,Giometto R,Höpner S,Centonze D,Bernardi G,Dell'Acqua ML,Rossini PM,Battistini L,Rötzschke O,Falk K||Blood (110:1225)||2007|
Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.
MA1-84019 was used in flow cytometry to investigate the changes of cytokine expression in NK cells after interacting with tumor cells
|Das S,Varalakshmi C,Kumari AL,Patel M,Khar A||Cancer immunology, immunotherapy : CII (50:428)||2001|
|Not Applicable||Not Cited||
Identification of a monoclonal antibody specific for a murine T3 polypeptide.
MA1-84019 was used in flow cytometry, immunocytochemistry, and immunoprecipitation to identify T3-epsilon as a cell surface protein involved in the transduction of activation signals
|Leo O,Foo M,Sachs DH,Samelson LE,Bluestone JA||Proceedings of the National Academy of Sciences of the United States of America (84:1374)||1987|
|Not Applicable||5 µg/ml||
Hair loss and defective T- and B-cell function in mice lacking ORAI1.
MA1-84019 was used in blocking or activating experiment to characterize the physiological consequences of ORAI1 deficiency
|Gwack Y,Srikanth S,Oh-Hora M,Hogan PG,Lamperti ED,Yamashita M,Gelinas C,Neems DS,Sasaki Y,Feske S,Prakriya M,Rajewsky K,Rao A||Molecular and cellular biology (28:5209)||2008|
|Not Applicable||Not Cited||
T cell antigen receptor phosphorylation induced by an anti-receptor antibody.
MA1-84019 was used in blocking or activating experiment to elucidate how 145-2C11 treatment results in kinase activation and CD3 phosphorylation
|Samelson LE,O'Shea JJ,Luong H,Ross P,Urdahl KB,Klausner RD,Bluestone J||Journal of immunology (Baltimore, Md. : 1950) (139:2708)||1987|
Lack of L-selectin expression by cells transferring diabetes in NOD mice: insights into the mechanisms involved in diabetes prevention by Mel-14 antibody treatment.
MA1-84019 was used in flow cytometry to assess the role of L-selectin in the pathogenesis of autoimmune diabetes.
|Lepault F,Gagnerault MC,Faveeuw C,Bazin H,Boitard C||European journal of immunology (25:1502)||1995|