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Anti-mouse CD16 / 32 antibody helps to reduce non-specific binding by activity competing for the Fc gamma receptor III / II. Dot plot A. Anti-mouse Vb7 TCR-R-PE [cat.no. RM4404] and anti-mouse CD3-FITC [cat. no. HM3401] staining of mouse lymph node tissue, no blocking reagent added. Dot plot B. Anti-mouse Vb7 TCR anti-mouse CD3 antibody staining following incubation with a mouse CD16 / 32 antibody (clone: FCR-4G8).
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Syrian hamster / IgG|
|Immunogen||C6VL-BS cell lysate|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1-0.25 µg per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Active recruitment of DNA methyltransferases regulates interleukin 4 in thymocytes and T cells.
HM3401 was used in flow cytometry to investigate how T cells regulate interleukin 4 expression.
|Makar KW,Pérez-Melgosa M,Shnyreva M,Weaver WM,Fitzpatrick DR,Wilson CB||Nature immunology (4:1183)||2003|
CD3-epsilon; CD3e antigen; epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; T-cell antigen receptor complex; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE
AI504783; CD3; Cd3e; CD3epsilon; T3e