|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Syrian hamster / IgG|
|Immunogen||C6VL-BS cell lysate|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1-0.25 µg per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||0.5-10 µg/ml||
CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones.
HM3428 was used in blocking or activating experiment to evaluate if an antibody-CD28 co-stimulatory signal blocks anergy induction in T-cell clones in mice
|Harding FA,McArthur JG,Gross JA,Raulet DH,Allison JP||Nature (356:607)||1992|
|Not Applicable||Not Cited||
Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes.
HM3428 was used in flow cytometry to determine the function of CD4+8+ thymocytes in thymic differentiation
|Havran WL,Poenie M,Kimura J,Tsien R,Weiss A,Allison JP||Nature (330:170)||1987|