|Flow Cytometry (Flow)||1:50-1:100|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Many|
|Host / Isotype||Rat / IgG1|
|Immunogen||Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys)|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This antibody reacts with CD3 epsilon. Predicted to react with birds and all mammals based on sequence homology.
Heat-mediated antigen retrieval using Tris/EDTA (pH 9) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is tonsil.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: antigen CD3E, epsilon polypeptide (TiT3 complex); CD3 antigen epsilon; CD3 antigen, epsilon polypeptide; CD3 epsilon; CD3 Epsilon chain; CD3 epsilon subunit; CD3 molecule, epsilon polypeptide; CD3-epsilon; CD3e; CD3e antigen; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; T-cell antigen receptor complex; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex
Gene Aliases: AI504783; BOS_14337; CD3; CD3E; CD3epsilon; IMD18; T3E; TCRE
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