|Tested species reactivity||Human|
|Published species reactivity||Dog|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic 13-mer peptide corresponding to internal region of the epsilon chain of human CD3 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibodly is suitable for staining normal and neoplastic T cells in formalin fixed, paraffin embedded tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Extreme Beta-Cell Deficiency in Pancreata of Dogs with Canine Diabetes.
PA1-37282 was used in immunohistochemistry to describe the pathophysiology of canine diabetes
|Shields EJ,Lam CJ,Cox AR,Rankin MM,Van Winkle TJ,Hess RS,Kushner JA||PloS one (10:null)||2015|