|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFN¿- Thpp cells.
Q10054 was used in flow cytometry to investigate the anti-influenza responses of T cell subsets
|Deng N,Weaver JM,Mosmann TR||PloS one (9:null)||2014|
|Not Applicable||Not Cited||
Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function.
Q10054 was used in flow cytometry to characterize T cells after MVA85A vaccination
|Dintwe OB,Day CL,Smit E,Nemes E,Gray C,Tameris M,McShane H,Mahomed H,Hanekom WA,Scriba TJ||European journal of immunology (43:2409)||2013|
CD4+ T-cell responses among adults and young children in response to Streptococcus pneumoniae and Haemophilus influenzae vaccine candidate protein antigens.
Q10054 was used in flow cytometry to determine cytokine profiles of CD4(+) T-helper (h) cells in adults and young children vaccinated with the antigens PspA, PcpA, PhtD, PhtE, Ply, LytB of Streptococcus pneumonia and protein D and OMP26 of non-typeable Haemophilus influenza.
|Sharma SK,Roumanes D,Almudevar A,Mosmann TR,Pichichero ME||Vaccine (31:3090)||2013|
Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.
Q10054 was used in flow cytometry to study influenza-specific CD4 T cells respond to vaccination using Ki67 as a marker.
|Li X,Miao H,Henn A,Topham DJ,Wu H,Zand MS,Mosmann TR||Vaccine (30:4581)||2012|
CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.
Q10054 was used in flow cytometry to examine human cross-reactive T cells against a pandemic virus.
|Scheible K,Zhang G,Baer J,Azadniv M,Lambert K,Pryhuber G,Treanor JJ,Topham DJ||Vaccine (29:2159)||2011|