|Flow Cytometry (Flow)||1:10-1:25|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:25|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:25|
|Immunoprecipitation (IP)||Assay Dependent|
|Tested Species reactivity||Rat|
|Host / Isotype||Mouse / IgM|
|Immunogen||F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This clone is suitable for use on paraffin embedded material using an antigen-unmasking fluid. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD3 antigen delta polypeptide; CD3 antigen delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 delta; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma polypeptide; CD3-DELTA; CD3-epsilon; CD3-GAMMA; CD3d; CD3d antigen; CD3e antigen; CD3g; CD3g antigen; CD3g molecule; delta chain; delta polypeptide TiT3 complex; epsilon CD3-TCR complex; epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; gamma polypeptide TiT3 complex; OKT3; T-cell antigen receptor complex; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T3D; T3E; T3G; TCRE
Gene Aliases: Cd3d; Cd3g; T3d
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