|Tested species reactivity||Dog|
|Host / Isotype||Mixed / IgG1, IgG2a|
|Immunogen||Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-9, Canine Concanavilin A activated T-cell blasts|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 5% sucrose, 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD3e FITC reagent: IgG1 (mouse). CD4 RPE reagent: IgG2a (rat). Clone CA17.2A12 recognizes canine CD3 epsilon, a T cell differentiation antigen expressed on mature T lymphocytes. Clone YKIX 302.9 recognizes canine CD4, which is expressed by lymphocyte subsets and canine neutrophils.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Reconstitute with 1 ml distilled water. After reconstitution, store product undiluted at 4°C in the dark.
CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.