|Tested species reactivity||Dog|
|Host / Isotype||Mixed / IgG1|
|Immunogen||Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-9, Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes canine CD3 epsilon and CD8 cell surface antigens. Clone CA17.2A12 is a FITC-conjugated mouse IgG1 that recognizes canine CD3, a T cell differentiation antigen expressed on mature T lymphocytes. Clone YCATE 55.9 is an RPE-conjugated Rat IgG1 that recognizes canine CD8 epsilon, which is expressed by cytotoxic T cells. YCATE 55.9 binds to CD8 alpha.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.