|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A synthetic peptide corresponding to the cytoplasmic domain of the human T Cell Receptor zeta chain coupled to a protein carrier.|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Ectopically expressed PIR-B on T cells constitutively binds to MHC class I and attenuates T helper type 1 responses.
13-7700 was used in immunoprecipitation to investigate the role of PIR-B in T cell development
|Imada M,Masuda K,Satoh R,Ito Y,Goto Y,Matsuoka T,Endo S,Nakamura A,Kawamoto H,Takai T||International immunology (21:1151)||2009|
||CTLA-4 suppresses proximal TCR signaling in resting human CD4(+) T cells by inhibiting ZAP-70 Tyr(319) phosphorylation: a potential role for tyrosine phosphatases.||Guntermann C,Alexander DR||Journal of immunology (Baltimore, Md. : 1950) (168:4420)||2002|
|Mouse||Not Cited||A mouse with a loss-of-function mutation in the c-Cbl TKB domain shows perturbed thymocyte signaling without enhancing the activity of the ZAP-70 tyrosine kinase.||Thien CB,Scaife RM,Papadimitriou JM,Murphy MA,Bowtell DD,Langdon WY||The Journal of experimental medicine (197:503)||2003|
|Human||Not Cited||CTLA-4 suppresses proximal TCR signaling in resting human CD4(+) T cells by inhibiting ZAP-70 Tyr(319) phosphorylation: a potential role for tyrosine phosphatases.||Guntermann C,Alexander DR||Journal of immunology (Baltimore, Md. : 1950) (168:4420)||2002|
A cell surface ADP-ribosyltransferase modulates T cell receptor association and signaling.
13-7700 was used in immunoprecipitation and western blot to study the activity of ART-1 in T cells.
|Liu ZX,Yu Y,Dennert G||The Journal of biological chemistry (274:17399)||1999|