|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide corresponding to the 113 amino acid residue cytoplasmic domain of murine zeta.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 µg/ml|
|Flow Cytometry (Flow)||1:50-1:100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20241 detects TCR zeta chain in human and mouse samples.
MA1-20241 has been successfully used in ELISA, FACS, immunohistochemistry (frozen), immunoprecipitation, and Western blot procedures.
The MA1-20241 immunogen is a synthetic peptide corresponding to the 113 amino acid residue cytoplasmic domain of mouse zeta.
The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.