|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Mouse CTL clone V4 cells|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Development of thymic microenvironments in vitro is oxygen-dependent and requires permanent presence of T-cell progenitors.
MA1-10220 was used in flow cytometry to investigate factors that mediate induction and maintenance of the 3D configuration of the epithelial network in fetal thymic lobes in vitro.
|Germeraad WT,Kawamoto H,Itoi M,Jiang Y,Amagai T,Katsura Y,van Ewijk W||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (51:1225)||2003|
Commitment of common T/Natural killer (NK) progenitors to unipotent T and NK progenitors in the murine fetal thymus revealed by a single progenitor assay.
MA1-10220 was used in flow cytometry to describe a clonal system that supports the development of T and natural killer cells evenly.
|Ikawa T,Kawamoto H,Fujimoto S,Katsura Y||The Journal of experimental medicine (190:1617)||1999|
Emergence of T, B, and myeloid lineage-committed as well as multipotent hemopoietic progenitors in the aorta-gonad-mesonephros region of day 10 fetuses of the mouse.
MA1-10220 was used in flow cytometry to investigate the aorta-gonad-mesonephros region as a source of hemopoietic progenitors.
|Ohmura K,Kawamoto H,Fujimoto S,Ozaki S,Nakao K,Katsura Y||Journal of immunology (Baltimore, Md. : 1950) (163:4788)||1999|
Emergence of T cell progenitors without B cell or myeloid differentiation potential at the earliest stage of hematopoiesis in the murine fetal liver.
MA1-10220 was used in flow cytometry to assess if progenitors colonizing the thymus are T cell lineage restricted.
|Kawamoto H,Ohmura K,Fujimoto S,Katsura Y||Journal of immunology (Baltimore, Md. : 1950) (162:2725)||1999|
B7 blockade prevents activation-induced cell death of thymocytes.
MA1-10220 was used in flow cytometry to elucidate the role of B7 in thymic selection.
|Samoilova EB,Horton JL,Bassiri H,Zhang H,Linsley PS,Carding SR,Chen Y||International immunology (9:1663)||1997|
Extrathymic maturation of alpha beta T cells from hemopoietic stem cells.
MA1-10220 was used in flow cytometry to test if alpha beta T cells develop from hematopoietic stem cells in the absence of the thymus.
|Dejbakhsh-Jones S,Jerabek L,Weissman IL,Strober S||Journal of immunology (Baltimore, Md. : 1950) (155:3338)||1995|
Progression of T cell lineage restriction in the earliest subpopulation of murine adult thymus visualized by the expression of lck proximal promoter activity.
MA1-10220 was used in flow cytometry to study regulation of the lck in T cell lineage commitment.
|Shimizu C,Kawamoto H,Yamashita M,Kimura M,Kondou E,Kaneko Y,Okada S,Tokuhisa T,Yokoyama M,Taniguchi M,Katsura Y,Nakayama T||International immunology (13:105)||2001|