|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Storage buffer||PBS with BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The deacetylase Sirt1 is an essential regulator of Aire-mediated induction of central immunological tolerance.
MCD0417 was used in flow cytometry to determine the role of Sirt1 in regulating the expression of tissue-restricted antigens in medullary thymic epithelial cells.
|Chuprin A,Avin A,Goldfarb Y,Herzig Y,Levi B,Jacob A,Sela A,Katz S,Grossman M,Guyon C,Rathaus M,Cohen HY,Sagi I,Giraud M,McBurney MW,Husebye ES,Abramson J||Nature immunology (16:737)||2015|
Histamine H(3) receptor integrates peripheral inflammatory signals in the neurogenic control of immune responses and autoimmune disease susceptibility.
MCD0417 was used in flow cytometry to characterize Hrh3 polymorphisms between experimental allergic encephalomyelitis-susceptible and resistant mice.
|Krementsov DN,Wall EH,Martin RA,Subramanian M,Noubade R,Del Rio R,Mawe GM,Bond JP,Poynter ME,Blankenhorn EP,Teuscher C||PloS one (8:null)||2013|
Systemic lack of canonical histamine receptor signaling results in increased resistance to autoimmune encephalomyelitis.
MCD0417 was used in flow cytometry to investigate the contribution of histamine on the development of experimental allergic encephalomyelitis.
|Saligrama N,Case LK,del Rio R,Noubade R,Teuscher C||Journal of immunology (Baltimore, Md. : 1950) (191:614)||2013|
The neonatal CNS is not conducive for encephalitogenic Th1 T cells and B cells during experimental autoimmune encephalomyelitis.
MCD0417 was used in flow cytometry to test if a differential composition of immune cells within the central nervous system modulates the age-associated susceptibility to autoimmune disease.
|Cravens PD,Kieseier BC,Hussain R,Herndon E,Arellano B,Ben LH,Timmons BC,Castro-Rojas C,Hartung HP,Hemmer B,Weber MS,Zamvil SS,Stüve O||Journal of neuroinflammation (10:null)||2013|
Combinatorial roles for histamine H1-H2 and H3-H4 receptors in autoimmune inflammatory disease of the central nervous system.
MCD0417 was used in flow cytometry to test if combinatorial targeting of histamine receptors is an effective therapy for experimental allergic encephalomyelitis.
|Saligrama N,Noubade R,Case LK,del Rio R,Teuscher C||European journal of immunology (42:1536)||2012|
Histamine H4 receptor optimizes T regulatory cell frequency and facilitates anti-inflammatory responses within the central nervous system.
MCD0417 was used in flow cytometry to investigate the contribution of H(4)R to autoimmunity.
|del Rio R,Noubade R,Saligrama N,Wall EH,Krementsov DN,Poynter ME,Zachary JF,Thurmond RL,Teuscher C||Journal of immunology (Baltimore, Md. : 1950) (188:541)||2012|
Genetics of experimental allergic encephalomyelitis supports the role of T helper cells in multiple sclerosis pathogenesis.
MCD0417 was used in flow cytometry to identify genes involved in multiple sclerosis and experimental allergic encephalomyelitis.
|Blankenhorn EP,Butterfield R,Case LK,Wall EH,del Rio R,Diehl SA,Krementsov DN,Saligrama N,Teuscher C||Annals of neurology (70:887)||2011|
|Not Applicable||Not Cited||
Hematopoietic stem cell dose correlates with the speed of immune reconstitution after stem cell transplantation.
MCD0417 was used in flow cytometry to test if higher numbers of hematopoietic stem cells correlate with the speed of immune reconstitution in a congenic transplantation mouse model
|Chen BJ,Cui X,Sempowski GD,Domen J,Chao NJ||Blood (103:4344)||2004|
Histamine H¿ receptor signaling × environment interactions determine susceptibility to experimental allergic encephalomyelitis.
MCD0417 was used in flow cytometry to demonstrate that regulation of encephalitogenic T-cell responses and EAE susceptibility by H2R signaling in CD4(+) T cells is dependent on genetic and environmental interactions.
|Saligrama N,Case LK,Krementsov DN,Teuscher C||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (28:1898)||2014|