|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gestational hypothyroidism increases the severity of experimental autoimmune encephalomyelitis in adult offspring.
MCD0420 was used in immunohistochemistry to investigate the role of gestational hypothyroidism on the development of autoimmune disease.
|Albornoz EA,Carreño LJ,Cortes CM,Gonzalez PA,Cisternas PA,Cautivo KM,Catalán TP,Opazo MC,Eugenin EA,Berman JW,Bueno SM,Kalergis AM,Riedel CA||Thyroid : official journal of the American Thyroid Association (23:1627)||2013|
Unique requirements for reactivation of virus-specific memory B lymphocytes.
MCD0420 was used in immunocytochemistry to investigate factors that mediate memory B cells activation
|Weisel FJ,Appelt UK,Schneider AM,Horlitz JU,van Rooijen N,Korner H,Mach M,Winkler TH||Journal of immunology (Baltimore, Md. : 1950) (185:4011)||2010|
Extension of the germinal center stage of B cell development promotes autoantibodies in BXD2 mice.
MCD0420 was used in immunohistochemistry - frozen section to determine the mechanisms by which RGS13 promotes the generation of pathogenic autoantibodies in germinal centers.
|Wang JH,New JS,Xie S,Yang P,Wu Q,Li J,Luo B,Ding Y,Druey KM,Hsu HC,Mountz JD||Arthritis and rheumatism (65:2703)||2013|
|Not Applicable||Not Cited||
Marginal zone precursor B cells as cellular agents for type I IFN-promoted antigen transport in autoimmunity.
MCD0420 was used in immunohistochemistry to study type I IFN and its role in regulating the migration response of antigen delivery by B cells into lymphoid follicles in autoimmune mice
|Wang JH,Li J,Wu Q,Yang P,Pawar RD,Xie S,Timares L,Raman C,Chaplin DD,Lu L,Mountz JD,Hsu HC||Journal of immunology (Baltimore, Md. : 1950) (184:442)||2010|