|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Experimental cerebral malaria pathogenesis--hemodynamics at the blood brain barrier.
MCD0430 was used in flow cytometry to evaluate a model of cerebral malaria pathogenesis and its effect on the blood brain barrier
|Nacer A,Movila A,Sohet F,Girgis NM,Gundra UM,Loke P,Daneman R,Frevert U||PLoS pathogens (10:null)||2014|
Interleukin-4 regulates eomesodermin in CD8+ T cell development and differentiation.
MCD0430 was used in flow cytometry to examine the role of interleukin-4 in relation to eomesodermin within CD8+ T cells
|Carty SA,Koretzky GA,Jordan MS||PloS one (9:null)||2014|
Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis.
MCD0430 was used in flow cytometry to assess the contribution of Tbx1 to thymus and parathyroid development
|Reeh KA,Cardenas KT,Bain VE,Liu Z,Laurent M,Manley NR,Richie ER||Development (Cambridge, England) (141:2950)||2014|
Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation.
MCD0430 was used in flow cytometry to study the involvement of endothelium in efficient Treg T cell recruitment in vivo
|Fu H,Kishore M,Gittens B,Wang G,Coe D,Komarowska I,Infante E,Ridley AJ,Cooper D,Perretti M,Marelli-Berg FM||Nature communications (5:null)||2014|
IL-21 is a double-edged sword in the systemic lupus erythematosus-like disease of BXSB.Yaa mice.
MCD0430 was used in flow cytometry to generate mice that lack IL-2R on B cells, on all T cells, or on CD8(+) T cells alone and examine the effects on systemic lupus erythematosus-like autoimmune disease.
|McPhee CG,Bubier JA,Sproule TJ,Park G,Steinbuck MP,Schott WH,Christianson GJ,Morse HC,Roopenian DC||Journal of immunology (Baltimore, Md. : 1950) (191:4581)||2013|
MHC class I family proteins retard systemic lupus erythematosus autoimmunity and B cell lymphomagenesis.
MCD0430 was used in flow cytometry to determine the contributions of individual MHC class I family members to autoimmunity in two mouse models.
|McPhee CG,Sproule TJ,Shin DM,Bubier JA,Schott WH,Steinbuck MP,Avenesyan L,Morse HC,Roopenian DC||Journal of immunology (Baltimore, Md. : 1950) (187:4695)||2011|
Influence of natural killer cells and perforin¿mediated cytolysis on the development of chemically induced lung cancer in A/J mice.
MCD0430 was used in flow cytometry to determine which type of immune cells contributes to the protection from lung cancer development.
|Frese-Schaper M,Keil A,Yagita H,Steiner SK,Falk W,Schmid RA,Frese S||Cancer immunology, immunotherapy : CII (63:571)||2014|
Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells.
MCD0430 was used in flow cytometry to characterize mice deficient in podoplanin and explore the effect of podoplanin modulation on T cell populations.
|Fuertbauer E,Zaujec J,Uhrin P,Raab I,Weber M,Schachner H,Bauer M,Schütz GJ,Binder BR,Sixt M,Kerjaschki D,Stockinger H||Immunology letters (154:31)||2013|
A transgenic mouse model genetically tags all activated CD8 T cells.
MCD0430 was used in flow cytometry to track the dynamics of the antiviral CD8+ T cell responses using a transgenic mouse that marks activated T cells.
|Maris CH,Miller JD,Altman JD,Jacob J||Journal of immunology (Baltimore, Md. : 1950) (171:2393)||2003|
BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
MCD0430 was used in immunohistochemistry to investigate the role of BTB-ZF factors in lymphoid effector programs
|Mathew R,Seiler MP,Scanlon ST,Mao AP,Constantinides MG,Bertozzi-Villa C,Singer JD,Bendelac A||Nature (491:618)||2012|