|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human PHA blasts|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:10 -1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is human lymphatic tissue. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mature dendritic cells in inflamed human pulps beneath deep caries.
MA5-16936 was used in immunohistochemistry to test if the frequency of CD83+ mature DCs correlates with caries invasion
|Harmon MA,Tew JG,Best AM,Hahn CL||Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (107:727)||2009|
|Not Applicable||Not Cited||
Etiological treatment during early chronic indeterminate Chagas disease incites an activated status on innate and adaptive immunity associated with a type 1-modulated cytokine pattern.
MA5-16936 was used in flow cytometry to assess the immunological status after Bz-treatment during early indeterminate Chagas disease
|Sathler-Avelar R,Vitelli-Avelar DM,Massara RL,de Lana M,Pinto Dias JC,Teixeira-Carvalho A,Elói-Santos SM,Martins-Filho OA||Microbes and infection (10:103)||2008|
|Not Applicable||Not Cited||
Activation/modulation of adaptive immunity emerges simultaneously after 17DD yellow fever first-time vaccination: is this the key to prevent severe adverse reactions following immunization?
MA5-16936 was used in flow cytometry to characterize the kinetics of the adaptive immune response following primary 17DD vaccination in order to clarify how it elicits such broad protection
|Martins MA,Silva ML,Marciano AP,Peruhype-Magalhães V,Eloi-Santos SM,Ribeiro jG,Correa-Oliveira R,Homma A,Kroon EG,Teixeira-Carvalho A,Martins-Filho OA||Clinical and experimental immunology (148:90)||2007|
Expression of the B7-related molecule ICOSL by human glioma cells in vitro and in vivo.
MA5-16936 was used in flow cytometry to demonstrate the expression of ICOSL by glioma cells in vitro and in vivo.
|Schreiner B,Wischhusen J,Mitsdoerffer M,Schneider D,Bornemann A,Melms A,Tolosa E,Weller M,Wiendl H||Glia (44:296)||2003|