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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 3 publications below|
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Qualification of a whole blood intracellular cytokine staining assay to measure mycobacteria-specific CD4 and CD8 T cell immunity by flow cytometry.
Q10008 was used in flow cytometry to assess the effects of long-term cryopreservation of fixed cells on the measurement of mycobacteria induced CD4 T cell immunity
|Kagina BM,Mansoor N,Kpamegan EP,Penn-Nicholson A,Nemes E,Smit E,Gelderbloem S,Soares AP,Abel B,Keyser A,Sidibana M,Hughes JE,Kaplan G,Hussey GD,Hanekom WA,Scriba TJ||Journal of immunological methods (417:22)||2015|
The novel tuberculosis vaccine, AERAS-402, is safe in healthy infants previously vaccinated with BCG, and induces dose-dependent CD4 and CD8T cell responses.
Q10008 was used in flow cytometry to test the safety and immunogenicity of an adenovirus 35-vectored tuberculosis vaccine candidate AERAS-402 in infants
|Kagina BM,Tameris MD,Geldenhuys H,Hatherill M,Abel B,Hussey GD,Scriba TJ,Mahomed H,Sadoff JC,Hanekom WA,Mansoor N,Hughes J,de Kock M,Whatney W,Africa H,Krohn C,Veldsman A,Kany AL,Douoguih M,Pau MG,Hendriks J,McClainc B,Benko J,Snowden MA,Hokey DA||Vaccine (32:5908)||2014|
Quantitative and qualitative differences in the T cell response to HIV in uninfected Ugandans exposed or unexposed to HIV-infected partners.
Q10008 was used in flow cytometry to characterize T cell responses of HIV-exposed, yet persistently uninfected individuals.
|Pala P,Serwanga J,Watera C,Ritchie AJ,Moodie Z,Wang M,Goonetilleke N,Birabwa E,Hughes P,Senkaali D,Nakiboneka R,Grosskurth H,Haynes B,McMichael A,Kaleebu P||Journal of virology (87:9053)||2013|
CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3