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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/50 - 1/200|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD4 cell surface antigen is a 55kDa glycoprotein expressed by helper T cells and weakly by monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4.
MA1-82247 was used in flow cytometry to identify the initial source of IL-4 in early immune responses.
|Liu L,Rich BE,Inobe J,Chen W,Weiner HL||International immunology (10:1017)||1998|
Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice.
MA1-82247 was used in flow cytometry to characterize T cell populations in IL-7 -/- mice.
|Moore TA,von Freeden-Jeffry U,Murray R,Zlotnik A||Journal of immunology (Baltimore, Md. : 1950) (157:2366)||1996|
CD4; CD4 antigen p55; CD4 receptor; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
Cd4; L3T4; Ly-4