|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/50 - 1/200|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD4 cell surface antigen is a 55kDa glycoprotein expressed by helper T cells and weakly by monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Experimental Trypanosoma cruzi infection alters the shaping of the central and peripheral T-cell repertoire.
MA1-82247 was used in flow cytometry to examine thymic and peripheral T-lymphocyte subsets in mice infected with Trypanosoma cruzi infection.
|Mendes-da-Cruz DA,de Meis J,Cotta-de-Almeida V,Savino W||Microbes and infection (5:825)||2003|
Helper T cells regulate type-2 innate immunity in vivo.
MA1-82247 was used in flow cytometry to report that polarized type-2 immune responses are initiated independently of adaptive immunity.
|Shinkai K,Mohrs M,Locksley RM||Nature (420:825)||2002|
Roles of cytotoxic T-lymphocyte-associated antigen-4 in the inductive phase of oral tolerance.
MA1-82247 was used in flow cytometry to elucidate the roles of cytotoxic T-lymphocyte-associated antigen-4 in oral tolerance.
|Chen Y,Ma Y,Chen Y||Immunology (105:171)||2002|
IL-10 transgenic mice present a defect in T cell development reminiscent of SCID patients.
MA1-82247 was used in flow cytometry to report that IL-10 regulates T cell maturation.
|Rouleau M,Cottrez F,Bigler M,Antonenko S,Carballido JM,Zlotnik A,Roncarolo MG,Groux H||Journal of immunology (Baltimore, Md. : 1950) (163:1420)||1999|
Depletion of CD8+ cells abolishes memory in acquired immunity against Chlamydia pneumoniae in BALB/c mice.
MA1-82247 was used in flow cytometry to determine the importance of T cells in Chlamydia pneumoniae infection.
|Penttilä JM,Anttila M,Varkila K,Puolakkainen M,Sarvas M,Mäkelä PH,Rautonen N||Immunology (97:490)||1999|
Maturation of CD4+ lymphocytes in the aged microenvironment results in a memory-enriched population.
MA1-82247 was used in flow cytometry to test the effect of an aged microenvironment on the maturation of newly produced CD4+ T cells.
|Timm JA,Thoman ML||Journal of immunology (Baltimore, Md. : 1950) (162:711)||1999|
Genetic regulation of commitment to interleukin 4 production by a CD4(+) T cell-intrinsic mechanism.
MA1-82247 was used in flow cytometry to use T cells from BALB mice and study IL-4 production.
|Bix M,Wang ZE,Thiel B,Schork NJ,Locksley RM||The Journal of experimental medicine (188:2289)||1998|
Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4.
MA1-82247 was used in flow cytometry to identify the initial source of IL-4 in early immune responses.
|Liu L,Rich BE,Inobe J,Chen W,Weiner HL||International immunology (10:1017)||1998|
Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice.
MA1-82247 was used in flow cytometry to characterize T cell populations in IL-7 -/- mice.
|Moore TA,von Freeden-Jeffry U,Murray R,Zlotnik A||Journal of immunology (Baltimore, Md. : 1950) (157:2366)||1996|
Expression of a novel integrin beta 1 chain epitope and anti-beta 1 antibody-mediated enhancement of fibronectin binding are dependent on the stage of T cell differentiation.
MA1-82247 was used in flow cytometry to investigate KMI6 recognition of beta 1 integrins on T cells.
|Wadsworth SA,Chang AC,Hong MJ,Halvorson MJ,Otto S,Coligan JE||Journal of immunology (Baltimore, Md. : 1950) (154:2125)||1995|
CD3+ and CD4+ cells adoptively transfer experimental hypersensitivity pneumonitis.
MA1-82247 was used in flow cytometry to describe the cells that transfer adoptive murine experimental hypersensitivity pneumonitis.
|Schuyler M,Gott K,Shopp G,Crooks L||The American review of respiratory disease (146:1582)||1992|
|Not Applicable||Not Cited||
Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes.
MA1-82247 was used in flow cytometry to study PD-1 expression in normal murine lymphoid tissues
|Agata Y,Kawasaki A,Nishimura H,Ishida Y,Tsubata T,Yagita H,Honjo T||International immunology (8:765)||1996|