|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.2 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17443 targets CD4 in FACS applications and shows reactivity with Mouse samples.
The MA5-17443 immunogen is mouse thymocytes.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD27 (50 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily. CD40 and CD30 are also members of the TNF receptor superfamily. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is a membranebound receptor, but a soluble form of CD27 is also produced.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunologic memory induced by a glycoconjugate vaccine in a murine adoptive lymphocyte transfer model.
MA5-17443 was used in flow cytometry to develop a mouse model to study the ability of a glycoprotein conjugate vaccine to induce immunologic memory for the polysaccharide moiety.
|Guttormsen HK,Wetzler LM,Finberg RW,Kasper DL||Infection and immunity (66:2026)||1998|
Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells.
MA5-17443 was used in flow cytometry to elucidate the pathway and cytokine regulation of NK cell development in vitro.
|Aiba Y,Hirayama F,Ogawa M||Blood (89:4005)||1997|
CD3+ and CD4+ cells adoptively transfer experimental hypersensitivity pneumonitis.
MA5-17443 was used in flow cytometry to describe the cells that transfer adoptive murine experimental hypersensitivity pneumonitis.
|Schuyler M,Gott K,Shopp G,Crooks L||The American review of respiratory disease (146:1582)||1992|
|Not Applicable||Not Cited||
Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes.
MA5-17443 was used in flow cytometry to study PD-1 expression in normal murine lymphoid tissues
|Agata Y,Kawasaki A,Nishimura H,Ishida Y,Tsubata T,Yagita H,Honjo T||International immunology (8:765)||1996|