|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 7 publications below|
MA5-17445 targets CD4 in FACS applications and shows reactivity with Mouse samples.
The MA5-17445 immunogen is mouse thymocytes.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD27 (50 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily. CD40 and CD30 are also members of the TNF receptor superfamily. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is a membranebound receptor, but a soluble form of CD27 is also produced.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IL-18 enhances IL-4 production by ligand-activated NKT lymphocytes: a pro-Th2 effect of IL-18 exerted through NKT cells.
MA5-17445 was used in flow cytometry to investigate the effect of IL-18 on NKT cells.
|Leite-De-Moraes MC,Hameg A,Pacilio M,Koezuka Y,Taniguchi M,Van Kaer L,Schneider E,Dy M,Herbelin A||Journal of immunology (Baltimore, Md. : 1950) (166:945)||2001|
Genetic regulation of commitment to interleukin 4 production by a CD4(+) T cell-intrinsic mechanism.
MA5-17445 was used in flow cytometry to use T cells from BALB mice and study IL-4 production.
|Bix M,Wang ZE,Thiel B,Schork NJ,Locksley RM||The Journal of experimental medicine (188:2289)||1998|
Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4.
MA5-17445 was used in flow cytometry to identify the initial source of IL-4 in early immune responses.
|Liu L,Rich BE,Inobe J,Chen W,Weiner HL||International immunology (10:1017)||1998|
Surface antigen expression in spleen cells of C57B1/6 mice during ageing: influence of sex and parity.
MA5-17445 was used in flow cytometry to test if pregnancies and gender influence the cellar changes observed during ageing in mice.
|Barrat F,Lesourd BM,Louise A,Boulouis HJ,Vincent-Naulleau S,Thibault D,Sanaa M,Neway T,Pilet CH||Clinical and experimental immunology (107:593)||1997|
Exclusive development of T cell neoplasms in mice transplanted with bone marrow expressing activated Notch alleles.
MA5-17445 was used in flow cytometry to report that TAN1 is an oncoprotein.
|Pear WS,Aster JC,Scott ML,Hasserjian RP,Soffer B,Sklar J,Baltimore D||The Journal of experimental medicine (183:2283)||1996|
Pathogenic and protective roles of CD45RB(low) CD4+ cells correlate with cytokine profiles in the spontaneously autoimmune diabetic mouse.
MA5-17445 was used in flow cytometry to test if CD45RB(low) population of CD4+ cells is protective or pathogenic to the development of disease in the NOD mouse.
|Shimada A,Rohane P,Fathman CG,Charlton B||Diabetes (45:71)||1996|
Expression of a novel integrin beta 1 chain epitope and anti-beta 1 antibody-mediated enhancement of fibronectin binding are dependent on the stage of T cell differentiation.
MA5-17445 was used in flow cytometry to investigate KMI6 recognition of beta 1 integrins on T cells.
|Wadsworth SA,Chang AC,Hong MJ,Halvorson MJ,Otto S,Coligan JE||Journal of immunology (Baltimore, Md. : 1950) (154:2125)||1995|