|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b, kappa|
|Storage buffer||PBS with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 4 publications below|
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Development of thymic microenvironments in vitro is oxygen-dependent and requires permanent presence of T-cell progenitors.
A27063 was used in flow cytometry to investigate factors that mediate induction and maintenance of the 3D configuration of the epithelial network in fetal thymic lobes in vitro.
|Germeraad WT,Kawamoto H,Itoi M,Jiang Y,Amagai T,Katsura Y,van Ewijk W||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (51:1225)||2003|
Extensive proliferation of T cell lineage-restricted progenitors in the thymus: an essential process for clonal expression of diverse T cell receptor beta chains.
A27063 was used in flow cytometry to assess the proliferative potential of thymic progenitors that are T cell lineage restricted.
|Kawamoto H,Ohmura K,Fujimoto S,Lu M,Ikawa T,Katsura Y||European journal of immunology (33:606)||2003|
Reactive oxygen intermediates during programmed cell death induced in the thymus of the Ts(1716)65Dn mouse, a murine model for human Down's syndrome.
A27063 was used in flow cytometry to elucidate role of SOD-1 overexpression in thymocyte biology.
|Paz-Miguel JE,Flores R,Sánchez-Velasco P,Ocejo-Vinyals G,Escribano de Diego J,López de Rego J,Leyva-Cobián F||Journal of immunology (Baltimore, Md. : 1950) (163:5399)||1999|
Isolation of the most immature population of murine fetal thymocytes that includes progenitors capable of generating T, B, and myeloid cells.
A27063 was used in flow cytometry to report that FcR is a useful marker to isolate the most immature population of murine fetal thymic cells.
|Hattori N,Kawamoto H,Katsura Y||The Journal of experimental medicine (184:1901)||1996|