Histogram of gated human lymphocytes labeled with CD4 Mouse Anti-Human mAb (clone S3.5), Qdot® 800 Conjugate. Sample was acquired and analyzed using 405 nm excitation and a 780/60 band pass emission filter using a BD LSR II flow cytometer (BD Biosciences, San Jose, CA).
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Quantum dots thermal stability improves simultaneous phenotype-specific telomere length measurement by FISH-flow cytometry.
Q22153 was used in flow cytometry to study the improvement of simultaneous phenotype-specific telomere length measurement by FISH-flow cytometry by quantum dots thermal stability
|Kapoor V,Hakim FT,Rehman N,Gress RE,Telford WG||Journal of immunological methods (344:6)||2009|