|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human peripheral blood T lymphocytes and HPB-ALL T-cell line|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is a monoclonal mixture of FITC-conjugated CD4 (clone 7E14, isotype IgG1) and PE-conjugated CD28 (clone B-23, isotype IgG1).
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.