|Tested species reactivity||Human|
|Host / Isotype||Mixed / IgG1|
|Immunogen||CD4-stimulated human leukocytes, CD8-synthetic peptide containing the 13 C-terminal amino acids of the cytoplasmic domain of the a-chain of the CD8 molecule|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1:1-1:10|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This CD4/CD8 monoclonal cocktail contains Mouse HRP CD4 (Clone 4B12, IgG1 kappa) and Rabbit AP CD8 (Clone SP16, IgG). It is designed for qualitative immunohistochemistry with normal and neoplastic formalin-fixed, paraffin-embedded tissues using the MultiVision anti-mouse/HRP + anti-rabbit/AP detection system (Catalog # TL-012-MHRA). This cocktail is provided at an optimal concentration in an easy-to-use format for visualizing CD4 and CD8 lymphocyte subsets in red and blue, respectively. The use of rabbit monoclonal CD8 allows for superior staining, in both sensitivity and specificity, permiting the use of higher titers compared to mouse monoclonal antibodies. These cocktails are specially formulated from MultiVision polymers that provide increased sensitivity, time-savings, and detection simplicity. The MultiVision AP and HRP polymers are innovative, patented technology. The smaller amino acid polymer subunits minimize conflicts in binding the target protein resulting in more consistent staining and better signal amplification. Ultimately, this gives the user higher sensitivity and antibody efficiency as well as better signal-to-noise ratios. This system is biotin-free, which prevents background staining found with traditional biotin-based methods. The MultiVision polymer detection systems yield high quality double staining in less than 2 hours on formalin-fixed and paraffin-embedded tissue sections. Prior to staining, heat-induced epitope recovery (HIER) is recommended, using 10mM EDTA (pH 8.0) for 20min at 98°C followed by cooling at room temperature for 20min. The kits allow observation of two single-stained cells in red and blue and/or co-localization marked by a purple/brownish mixed-color.
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.