|Tested species reactivity||Dog|
|Host / Isotype||Rat / Cocktail|
|Immunogen||Canine Concanavilin A activated T-cell blasts, Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-16990 contains a monoclonal mixture of FITC-conjugated CD4 (clone #YKIX 302.9, Rat IgG2a) and RPE-conjugated CD8 (clone #YCATE 55.9, Rat IgG1). This antibody is a dual-color reagent recognizing canine CD4 and CD8 cell surface antigens. Clone YKIX 302.9 recognizes canine CD4, which is expressed by lymphocyte subsets and canine neutrophils. Clone YCATE 55.9 recognizes CD8 alpha, which is expressed by cytotoxic T cells.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.