|Tested species reactivity||Mouse|
|Host / Isotype||Rat / Cocktail|
|Immunogen||T cell clone, C6|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17009 contains a monoclonal mixture of FITC-conjugated CD4 (clone #YTS191.1, Rat IgG2b) and RPE-conjugated CD8 (clone #KT15, Rat IgG2a). This antibody is a dual-color reagent recognizing mouse CD4 and CD8 cell surface antigens. Clone YTS191.1 recognizes murine CD4, a T cell differentiation antigen expressed on thymocytes and helper/inducer T cells in the peripheral blood. Clone KT15 recognizes a non-polymorphic epitope on the murine CD8 alpha chain.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.