|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Extracellular domain of Murine CD40 and the Fc portion of Human IgG1.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1/50|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The CD40 antigen is a single chain glycoprotein. It is known to be a member of the tumor necrosis factor / nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells. It is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. The precise function of the CD40 antigen is unknown but it appears to be involved in the transduction of regulatory signals for cellular functions such as B cell proliferation and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Purified eicosapentaenoic acid induces prolonged survival of cardiac allografts and generates regulatory T cells.
MA1-81395 was used in flow cytometry to test if eicosapentaenoic acid treatment alters the survival of fully mismatched murine cardiac allografts
|Iwami D,Zhang Q,Aramaki O,Nonomura K,Shirasugi N,Niimi M||American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons (9:1294)||2009|
|Not Applicable||Not Cited||
Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors.
MA1-81395 was used in flow cytometry to clone and characterize Siglec-H
|Zhang J,Raper A,Sugita N,Hingorani R,Salio M,Palmowski MJ,Cerundolo V,Crocker PR||Blood (107:3600)||2006|
A transmembrane-anchored rat RAE-1-like transcript as a ligand for NKR-P2, the rat ortholog of human and mouse NKG2D.
MA1-81395 was used in flow cytometry to identify the ligand for the rat ortholog of human and mouse NKG2D
|Savithri B,Khar A||European journal of immunology (36:107)||2006|
Activation of dendritic cells and induction of T cell responses by HPV 16 L1/E7 chimeric virus-like particles are enhanced by CpG ODN or sorbitol.
MA1-81395 was used in flow cytometry to study the interaction of human papillomavirus 16 L1/E7 chimeric virus-like particles with mouse bone marrow-derived dendritic cells activated with different immune adjuvants
|Freyschmidt EJ,Alonso A,Hartmann G,Gissmann L||Antiviral therapy (9:479)||2004|
Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.
MA1-81395 was used in flow cytometry to investigate the changes of cytokine expression in NK cells after interacting with tumor cells
|Das S,Varalakshmi C,Kumari AL,Patel M,Khar A||Cancer immunology, immunotherapy : CII (50:428)||2001|
Interleukin-2-induced nitric oxide synthase and nuclear factor-kappaB activity in activated natural killer cells and the production of interferon-gamma.
MA1-81395 was used in flow cytometry to investigate the role of interleukin 2 in the regulation of natural killer cell function
|Jyothi MD,Khar A||Scandinavian journal of immunology (52:148)||2000|
Target-cell-induced anergy in natural killer cells: suppression of cytotoxic function.
MA1-81395 was used in flow cytometry to investigate the cytotoxic functions of natural killer cells
|Das S,Varalakshmi C,Khar A||Cancer immunology, immunotherapy : CII (49:109)||2000|
Administration of anti-IL-12 antibody in vivo inhibits rejection of a rat histiocytoma and suppresses cytokine response in a tumour-bearing host.
MA1-81395 was used in flow cytometry to investigate the effect of interleukin 12 inhibition on tumor rejection
|Rao KL,Varalakshmi C,Ali AM,Khar A||Immunology (92:381)||1997|
Natural killer cell activation-associated induction of target cell DNA fragmentation in a spontaneously regressing rat histiocytoma.
MA1-81395 was used in flow cytometry to investigate the effect of natural killer cells on apoptosis in a regressing histiocytoma
|Bright JJ,Kausalya S,Khar A||Immunology (85:638)||1995|
Protease-activated receptor 1 activation is necessary for monocyte chemoattractant protein 1-dependent leukocyte recruitment in vivo.
MA1-81395 was used in immunocytochemistry to study the role of PAR activation in vivo to initiate MCP-dependent leukocyte recruitment
|Chen D,Carpenter A,Abrahams J,Chambers RC,Lechler RI,McVey JH,Dorling A||The Journal of experimental medicine (205:1739)||2008|
Passive transfer of flt-3L-derived dendritic cells delays diabetes development in NOD mice and associates with early production of interleukin (IL)-4 and IL-10 in the spleen of recipient mice.
MA1-81395 was used in flow cytometry to assess the contribution of DC function in non-obese diabetic mice.
|Morin J,Faideau B,Gagnerault MC,Lepault F,Boitard C,Boudaly S||Clinical and experimental immunology (134:388)||2003|
Uptake and presentation of antigen to T cells by primary colonic epithelial cells in normal and diseased states.
MA1-81395 was used in flow cytometry to determine the immunoregulatory properties of primary colonic epithelial cells.
|Telega GW,Baumgart DC,Carding SR||Gastroenterology (119:1548)||2000|
Dendritic cells in the induction of protective and nonprotective anticryptococcal cell-mediated immune responses.
MA1-81395 was used in flow cytometry to examine the contribution of different DC subsets during infection with Cryptococcus neoformans.
|Bauman SK,Nichols KL,Murphy JW||Journal of immunology (Baltimore, Md. : 1950) (165:158)||2000|
Efficient suicide gene therapy of transduced and distant untransduced ovary tumors is correlated with significant increase of intratumoral T and NK cells.
MA1-81395 was used in immunohistochemistry to study the mechanism for the suicide gene therapy of ovary tumors
|Nagy HJ,Panis Y,Fabre M,Engelmann C,Soubrane O,Houssin D,Klatzmann D||Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie (54:479)||2000|
Eradication of rat malignant gliomas by retroviral-mediated, in vivo delivery of the interleukin 4 gene.
MA1-81395 was used in immunohistochemistry to evaluate the interleukin 4 gene therapy of rat malignant gliomas
|Benedetti S,Bruzzone MG,Pollo B,DiMeco F,Magrassi L,Pirola B,Cirenei N,Colombo MP,Finocchiaro G||Cancer research (59:645)||1999|
Phospholipase A2-mediated inflammation induces regression of malignant gliomas.
MA1-81395 was used in immunohistochemistry to investigate the effect of phospholipase A2 on glioma inhibition in vivo
|Goddard DH,Bomalaski JS,Lipper S,Shorr RG,Clark MA||Cancer letters (102:1)||1996|
Linomide inhibits angiogenesis, growth, metastasis, and macrophage infiltration within rat prostatic cancers.
MA1-81395 was used in immunohistochemistry to study the role of mitochondrial pathway in neutrophil survival modulated by IgE in asthma
|Vukanovic J,Isaacs JT||Cancer research (55:1499)||1995|