|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human CD40.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil or Hodgkin's lymphoma.
The CD40 antigen is a single chain glycoprotein. It is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin and quote;s disease-associated antigen, CD30. CD40 is present on all B cells except plasma cells. It is also found on some epithelial cells, carcinomas and lymphoid dentritic cells. It appears that the CD40 antigen is involved in the transduction of regulatory signals for cellular functions such as B cell proliferation and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.