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Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or plate-bound hamster anti-mouse CD3e (clone 145-2C11) for 7 hours at 37°C. The cells were then harvested, double-stained with anti-CD154/CD40L-FITC and anti-CD4+8-R-PE, and analyzed by flow cytometry.
|Tested species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG, kappa|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1-0.2 ug per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD154; CD40 ligand; Cd40l; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; RP23-153G22.3; T-BAM; T-cell antigen Gp39; TNF-related activation protein; Tnfsf5; TRAP; tumor necrosis factor ligand superfamily member 5
CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP