Proteintech

CD44 Monoclonal Antibody (6F4H2)

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Antibody Testing Data (18)

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CD44 Antibody (60224-1-IG) in WB

HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with (Product # 60224-1-Ig) CD44 antibody at dilution of 1:5,000 incubated at room temperature for 1.5 hours.

Product Image Gallery

CD44 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells using 60224-1-IG (CD44 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

CD44 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CD44 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed Human lung cancer tissue using (Product # 60224-1-Ig) CD44 antibody at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CD44 Antibody in Flow Cytometry (Flow) — 1X10^6 Raji cells were stained with 0.2ug CD44 antibody (60224-1-IG, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-mouse IGG with dilution 1:1000.

Product Details

60224-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:10-1:100

View 17 publications 17 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Pig

Published species

Human, Mouse, Pig

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6F4H2

Immunogen

CD44 Fusion Protein Ag7633 (28-380 aa encoded by BC004372)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.27 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: CRFAGVFHV EKNGRYSISR TEAADLCKAF NSTLPTMAQM EKALSIGFET CRYGFIEGHV VIPRIHPNSI CAANNTGVYI LTSNTSQYDT YCFNASAPPE EDCTSVTDLP NAFDGPITIT IVNRDGTRYV QKGEYRTNPE DIYPSNPTDD DVSSGSSSER SSTSGGYIFY TFSTVHPIPD EDSPWITDST DRIPATSTSS NTISAGWEPN EENEDERDRH LSFSGSGIDD DEDFISSTIS TTPRAFDHTK QNQDWTQWNP SHSNPEVLLQ TTTRMTDVDR NGTTAYEGNW NPEAHPPLIH HEHHEEEETP HSTSTIQATP SSTTEETATQ KEQWFGNRWH EGYRQTPRED SHST (28-380 aa encoded by BC004372)

Target Information

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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The risk of using monoclonal or polyclonal commercial antibodies: controversial results on porcine sperm CD44 receptor identification.

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Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa.

The Journal of reproduction and development

Álvarez-Rodriguez M,Vicente-Carrillo A,Rodriguez-Martinez H

Mon Aug 20 00:00:00 EDT 2018

Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa.

The Journal of reproduction and development

Álvarez-Rodriguez M,Vicente-Carrillo A,Rodriguez-Martinez H

Mon Aug 20 00:00:00 EDT 2018

Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa.

The Journal of reproduction and development

Álvarez-Rodriguez M,Vicente-Carrillo A,Rodriguez-Martinez H

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Hydrogel Encapsulation Facilitates Rapid-Cooling Cryopreservation of Stem Cell-Laden Core-Shell Microcapsules as Cell-Biomaterial Constructs.

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Fri Dec 01 00:00:00 EST 2017

Hydrogel Encapsulation Facilitates Rapid-Cooling Cryopreservation of Stem Cell-Laden Core-Shell Microcapsules as Cell-Biomaterial Constructs.

Advanced healthcare materials

Zhao G,Liu X,Zhu K,He X

Fri Dec 01 00:00:00 EST 2017

Hydrogel Encapsulation Facilitates Rapid-Cooling Cryopreservation of Stem Cell-Laden Core-Shell Microcapsules as Cell-Biomaterial Constructs.

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Zhao G,Liu X,Zhu K,He X

Fri Dec 01 00:00:00 EST 2017

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Synthesis and characterization of a bimodal nanoparticle based on the host-guest self-assembly for targeted cellular imaging.

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Bufalin reverses acquired drug resistance by inhibiting stemness in colorectal cancer cells.

Oncology reports

Sun J,Xu K,Qiu Y,Gao H,Xu J,Tang Q,Yin P

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