Proteintech

CD44 Monoclonal Antibody (6F4H2)

CD44 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

CD44 Antibody (60224-1-IG) in ICC/IF

Immunofluorescent analysis of MCF-7 cells using 60224-1-IG (CD44 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

CD44 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD44 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Product Details

60224-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:10-1:100

View 17 publications 17 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Pig

Published species

Human, Mouse, Pig

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6F4H2

Immunogen

CD44 Fusion Protein Ag7633 (28-380 aa encoded by BC004372)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.27 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: CRFAGVFHV EKNGRYSISR TEAADLCKAF NSTLPTMAQM EKALSIGFET CRYGFIEGHV VIPRIHPNSI CAANNTGVYI LTSNTSQYDT YCFNASAPPE EDCTSVTDLP NAFDGPITIT IVNRDGTRYV QKGEYRTNPE DIYPSNPTDD DVSSGSSSER SSTSGGYIFY TFSTVHPIPD EDSPWITDST DRIPATSTSS NTISAGWEPN EENEDERDRH LSFSGSGIDD DEDFISSTIS TTPRAFDHTK QNQDWTQWNP SHSNPEVLLQ TTTRMTDVDR NGTTAYEGNW NPEAHPPLIH HEHHEEEETP HSTSTIQATP SSTTEETATQ KEQWFGNRWH EGYRQTPRED SHST (28-380 aa encoded by BC004372)

Target Information

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Wed Mar 01 00:00:00 UTC 2023

Connective tissue research

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Wed Mar 01 00:00:00 UTC 2023

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Nature communications

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Decellularised spinal cord matrix manipulates glial niche into repairing phase via serglycin-mediated signalling pathway.

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A histological study of vascular wall resident stem cells in venous malformations.

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Tue Nov 01 00:00:00 UTC 2022

A histological study of vascular wall resident stem cells in venous malformations.

Cell and tissue research

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Tue Nov 01 00:00:00 UTC 2022

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma.

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Tue Dec 27 00:00:00 UTC 2022

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma.

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Huang H,Huang F,Liang X,Fu Y,Cheng Z,Huang Y,Chen Z,Duan Y,Chen Y

Tue Dec 27 00:00:00 UTC 2022

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma.

Pharmaceuticals (Basel, Switzerland)

Huang H,Huang F,Liang X,Fu Y,Cheng Z,Huang Y,Chen Z,Duan Y,Chen Y

Tue Dec 27 00:00:00 UTC 2022

Afatinib Reverses EMT via Inhibiting CD44-Stat3 Axis to Promote Radiosensitivity in Nasopharyngeal Carcinoma.

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Journal of translational medicine

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Tue Jun 07 00:00:00 UTC 2022

Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.

Journal of translational medicine

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Tue Jun 07 00:00:00 UTC 2022

Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.

Journal of translational medicine

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Knockdown of ALDH1A3 reduces breast cancer stem cell marker CD44 via the miR-7-TGFBR2-Smad3-CD44 regulatory axis.

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Fri Oct 01 00:00:00 UTC 2021

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Fri Dec 17 00:00:00 UTC 2021

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All-Aqueous-Phase Microfluidics for Cell Encapsulation.

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Wed Feb 06 00:00:00 UTC 2019

The risk of using monoclonal or polyclonal commercial antibodies: controversial results on porcine sperm CD44 receptor identification.

Reproduction in domestic animals = Zuchthygiene

Alvarez-Rodriguez M,López-Bejar M,Rodriguez-Martinez H

Mon Apr 01 00:00:00 UTC 2019

The risk of using monoclonal or polyclonal commercial antibodies: controversial results on porcine sperm CD44 receptor identification.

Reproduction in domestic animals = Zuchthygiene

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Mon Apr 01 00:00:00 UTC 2019

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Theriogenology

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Sun Dec 01 00:00:00 UTC 2019

Vitrification of stem cell-laden core-shell microfibers with unusually low concentrations of cryoprotective agents.

Biomaterials science

Tian C,Zhang X,Zhao G

Tue Feb 26 00:00:00 UTC 2019

The Unusual Properties of Polytetrafluoroethylene Enable Massive-Volume Vitrification of Stem Cells with Low-Concentration Cryoprotectants.

Advanced materials technologies

Cao Y,Zhao G,Panhwar F,Zhang X,Chen Z,Cheng L,Zang C,Liu F,Zhao Y,He X

Tue Jan 01 00:00:00 UTC 2019

Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa.

The Journal of reproduction and development

Álvarez-Rodriguez M,Vicente-Carrillo A,Rodriguez-Martinez H

Mon Aug 20 00:00:00 UTC 2018

Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa.

The Journal of reproduction and development

Álvarez-Rodriguez M,Vicente-Carrillo A,Rodriguez-Martinez H

Mon Aug 20 00:00:00 UTC 2018

Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa.

The Journal of reproduction and development

Álvarez-Rodriguez M,Vicente-Carrillo A,Rodriguez-Martinez H

Mon Aug 20 00:00:00 UTC 2018

Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa.

The Journal of reproduction and development

Álvarez-Rodriguez M,Vicente-Carrillo A,Rodriguez-Martinez H

Mon Aug 20 00:00:00 UTC 2018

Magnetothermal heating facilitates the cryogenic recovery of stem cell-laden alginate-Fe₃O₄ nanocomposite hydrogels.

Biomaterials science

Zhang X,Zhao G,Cao Y,Haider Z,Wang M,Fu J

Tue Nov 20 00:00:00 UTC 2018

Hydrogel Encapsulation Facilitates Rapid-Cooling Cryopreservation of Stem Cell-Laden Core-Shell Microcapsules as Cell-Biomaterial Constructs.

Advanced healthcare materials

Zhao G,Liu X,Zhu K,He X

Fri Dec 01 00:00:00 UTC 2017

Hydrogel Encapsulation Facilitates Rapid-Cooling Cryopreservation of Stem Cell-Laden Core-Shell Microcapsules as Cell-Biomaterial Constructs.

Advanced healthcare materials

Zhao G,Liu X,Zhu K,He X

Fri Dec 01 00:00:00 UTC 2017

Hydrogel Encapsulation Facilitates Rapid-Cooling Cryopreservation of Stem Cell-Laden Core-Shell Microcapsules as Cell-Biomaterial Constructs.

Advanced healthcare materials

Zhao G,Liu X,Zhu K,He X

Fri Dec 01 00:00:00 UTC 2017

Synthesis and characterization of a bimodal nanoparticle based on the host-guest self-assembly for targeted cellular imaging.

Talanta

Wang H,Sun D,Liao H,Wang Y,Zhao S,Zhang Y,Lv G,Ma X,Liu Y,Sun G

Tue Aug 15 00:00:00 UTC 2017

Synthesis and characterization of a bimodal nanoparticle based on the host-guest self-assembly for targeted cellular imaging.

Talanta

Wang H,Sun D,Liao H,Wang Y,Zhao S,Zhang Y,Lv G,Ma X,Liu Y,Sun G

Tue Aug 15 00:00:00 UTC 2017

Bufalin reverses acquired drug resistance by inhibiting stemness in colorectal cancer cells.

Oncology reports

Sun J,Xu K,Qiu Y,Gao H,Xu J,Tang Q,Yin P

Fri Sep 01 00:00:00 UTC 2017

Bufalin reverses acquired drug resistance by inhibiting stemness in colorectal cancer cells.

Oncology reports

Sun J,Xu K,Qiu Y,Gao H,Xu J,Tang Q,Yin P

Fri Sep 01 00:00:00 UTC 2017

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Biochemical and biophysical research communications