|Flow Cytometry (Flow)||2 ug/test|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||See 5 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Immunocytochemistry (ICC)||See 5 publications below|
|Flow Cytometry (Flow)||See 4 publications below|
|Western Blot (WB)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|Immunohistochemistry (IHC)||See 36 publications below|
|Tested Species reactivity||Baboon, Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Stimulated human leukocytes|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13890 targets CD44 in FACS, IHC (P), ICC/IF, IP and WB applications and shows reactivity with Human, Baboon and Non-human primate samples.
The MA5-13890 immunogen is stimulated human leukocytes.
MA5-13890 was used in the immunofluorescence analysis to successfully detect CD44 in human mesenchymal stem cells derived from iPSCs.
CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), Phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing, and in tumor metastasis. Ab-4 reacts with red cells pretreated with 2-aminoethylisothiouronium bromide (AET). Digestion of intact erythrocytes by trypsin treatment leaves a 50-54kDa protein fragment attached to the membrane, while chymotrypsin leaves a somewhat similarly sized 51-56kDa component that are recognized by Ab-4. This antibody enhances IL-2 production by CD2-transfected murine hybridoma stimulated by JY cells while it has no significant effect on IL-2 production by Jurkat cells stimulated with CD3, PMA, or CD2. Ab-4 enhances proliferation of PBL stimulated with CD2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD44; CD44 antigen; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing adhesion receptor; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronate binding protein; Hyaluronate receptor; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; soluble CD44
Gene Aliases: CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1
UniProt ID: (Human) Q16208
Entrez Gene ID: (Human) 960
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