Immunofluorescent analysis of CD44 (green) showing staining in the membrane and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD44 monoclonal antibody (Product # MA4405) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Mouse 80-95 kD lymphocyte surface glycoprotein H-CAM (CD44).|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA4405 targets CD44 in FACS, IA, IF, IP, and WB applications and shows reactivity with mouse and Human samples.
The MA4405 immunogen is mouse 80-95 kD lymphocyte surface glycoprotein H-CAM (CD44).
MA4405 detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This product is a Low Endotoxin formulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice.
MA4405 was used in flow cytometry to determine the role for DOCK8 in peripheral CD8 T cell survival and function
|Randall KL,Chan SS,Ma CS,Fung I,Mei Y,Yabas M,Tan A,Arkwright PD,Al Suwairi W,Lugo Reyes SO,Yamazaki-Nakashimada MA,Garcia-Cruz Mde L,Smart JM,Picard C,Okada S,Jouanguy E,Casanova JL,Lambe T,Cornall RJ,Russell S,Oliaro J,Tangye SG,Bertram EM,Goodnow CC||The Journal of experimental medicine (208:2305)||2011|
Resistance to murine AIDS in offspring of mice infected with LP-BM5. Role of CD8 T cells.
MA4405 was used in flow cytometry to investigate the role of CD8 T cells in immune responses against murine leukemia viruses
|Pavlovitch JH,Hulier E,Rizk-Rabin M,Marussig M,Mazier D,Joffret ML,Hoos S,Papiernik M||Journal of immunology (Baltimore, Md. : 1950) (156:4757)||1996|
Intervention of CD4+ cell subset shifts and autoimmunity in the BXSB mouse by murine CTLA4Ig.
MA4405 was used in flow cytometry to investigate the effect of CTLA4lg treatment on autoimmune disease in the BXSB mouse
|Chu EB,Hobbs MV,Wilson CB,Romball CG,Linsley PS,Weigle WO||Journal of immunology (Baltimore, Md. : 1950) (156:1262)||1996|
Distinct phenotypes of antigen-selected CD8 T cells emerge at different stages of an in vivo immune response.
MA4405 was used in flow cytometry to investigate the T cell differentiation and maturation after immunization
|Walker PR,Ohteki T,Lopez JA,MacDonald HR,Maryanski JL||Journal of immunology (Baltimore, Md. : 1950) (155:3443)||1995|
Lymphoid environment limits superantigen and antigen-induced T cell proliferation at high precursor frequency.
MA4405 was used in flow cytometry to report that the lymphoid environment limits T cell proliferation in response to high superantigen levels.
|Attinger A,MacDonald HR,Acha-Orbea H||European journal of immunology (31:884)||2001|
Genetic characterization of a polymorphic murine cell-surface glycoprotein.
MA4405 was used in immunocytochemistry to characterize a murine cell-surface glycoprotein Pgp-1
|Lesley J,Trowbridge IS||Immunogenetics (15:313)||1982|