Immunofluorescent analysis of CD44 (red) in human bone marrow derived mesenchymal stem cells. The cells were fixed with 2% paraformaldehyde, and blocked with 2% normal donkey serum diluted in PBS for 30 minutes at room temperature. Cells were stained with a CD44 monoclonal antibody (Product # MA5-12394) at a dilution of 10ug/ml in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate (Product # A21422 ) at a dilution of 1:800 for 1 hour at room temperature (red). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Note: Data courtesy of Innovators Program.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||EBV transformed normal human B-lymphocytes|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||10 µg/mL|
|Immunofluorescence (IF)||10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12394 has been successfully used in immunofluorescence analysis of CD44 in human bone marrow derived mesenchymal stem cells.
CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), Phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing, and in tumor metastasis. Ligand for CD44 is hyaluronan of extracellular matrix. Ab-2 blocks the binding of hyaluronic acid to CD44 receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD44 mediated hyaluronan adhesion of Toxoplasma gondii-infected leukocytes.
MA5-12394 was used in blocking or activating experiment to study the role of elevated CD44 expression in the mechanism by which Toxoplasma gondii infection promotes increased leukocyte hyaluron adhesion
|Hayashi T,Unno A,Baba M,Ohno T,Kitoh K,Takashima Y||Parasitology international (63:479)||2014|
Hyaluronan initiates chondrogenesis mainly via CD44 in human adipose-derived stem cells.
MA5-12394 was used in blocking or activating experiment to study the role of the hyaluron-CD44 interaction in triggering chondrogenesis in human adipose tissue -derived stem cells
|Wu SC,Chen CH,Chang JK,Fu YC,Wang CK,Eswaramoorthy R,Lin YS,Wang YH,Lin SY,Wang GJ,Ho ML||Journal of applied physiology (Bethesda, Md. : 1985) (114:1610)||2013|
High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1.
MA5-12394 was used in blocking or activating experiment to study the effect high molecular weight hyaluronic acid on MMP production from human chondrocytes and its mechanism
|Hashizume M,Mihara M||Biochemical and biophysical research communications (403:184)||2010|
Adhesion-mediated self-renewal abilities of Ph+ blastoma cells.
MA5-12394 was used in blocking or activating experiment to investigate the effect of cell adhesion on leukemic stem cell self-renewal
|Funayama K,Saito-Kurimoto Y,Ebihara Y,Shimane M,Nomura H,Tsuji K,Asano S||Biochemical and biophysical research communications (396:193)||2010|
High molecular weight hyaluronic acid down-regulates the gene expression of osteoarthritis-associated cytokines and enzymes in fibroblast-like synoviocytes from patients with early osteoarthritis.
MA5-12394 was used in blocking or activating experiment to examine the therapeutic effect of high molecular weight hyaluronic acid in patients with early osteoarthritis
|Wang CT,Lin YT,Chiang BL,Lin YH,Hou SM||Osteoarthritis and cartilage (14:1237)||2006|
Suppressive effects of hyaluronan on MMP-1 and RANTES production from chondrocytes.
MA5-12394 was used in blocking or activating experiment to study the effects of hyaluronan on MMP-1 and RANTES production from chondrocytes
|Tanaka M,Masuko-Hongo K,Kato T,Nishioka K,Nakamura H||Rheumatology international (26:185)||2006|
Differentiation of human mesenchymal stem cells: the potential mechanism for estrogen-induced preferential osteoblast versus adipocyte differentiation.
MA5-12394 was used in flow cytometry to investigate the regulatory role of estrogen signaling pathways in the differentiation of mesenchymal stem cells in vitro
|Zhao JW,Gao ZL,Mei H,Li YL,Wang Y||The American journal of the medical sciences (341:460)||2011|
Inhibition of CD44 N- and O-linked glycosylation decreases endometrial cell lines attachment to peritoneal mesothelial cells.
MA5-12394 was used in flow cytometry to study the role of N- and O-linked glycosylation in the attachment of endothelial cells to peritoneal mesothelial cells.
|Rodgers AK,Nair A,Binkley PA,Tekmal R,Schenken RS||Fertility and sterility (95:823)||2011|
Inhibition of human pancreatic cell line MIA PaCa2 proliferation by HA-But, a hyaluronic butyric ester: a preliminary report.
MA5-12394 was used in flow cytometry to examine the effect of hyaluronic butyric ester HA-But on the proliferation of human pancreatic cell line MIA PaCa2
|Pellizzaro C,Speranza A,Zorzet S,Crucil I,Sava G,Scarlata I,Cantoni S,Fedeli M,Coradini D||Pancreas (36:e15)||2008|
[Mesenchymal stem cells transduced by PLEGFP-N1 retroviral vector maintain their biological features and differentiation].
MA5-12394 was used in flow cytometry to evaluate the utility of enhanced green fluorescent protein transduction in human mesenchymal stem cells
|He X,Li YL,Wang XR,Guo X,Niu Y||Chinese medical journal (118:1728)||2005|
Hyaluronic-acid butyric esters as promising antineoplastic agents in human lung carcinoma: a preclinical study.
MA5-12394 was used in flow cytometry to investigate the therapeutic effect of hyaluronic-acid butyric esters in lung carcinomas
|Coradini D,Pellizzaro C,Abolafio G,Bosco M,Scarlata I,Cantoni S,Stucchi L,Zorzet S,Turrin C,Sava G,Perbellini A,Daidone MG||Investigational new drugs (22:207)||2004|
Inhibition of hepatocellular carcinomas in vitro and hepatic metastases in vivo in mice by the histone deacetylase inhibitor HA-But.
MA5-12394 was used in flow cytometry to investigate the effect of histone deacetylase inhibitor on intrahepatic tumor lesions
|Coradini D,Zorzet S,Rossin R,Scarlata I,Pellizzaro C,Turrin C,Bello M,Cantoni S,Speranza A,Sava G,Mazzi U,Perbellini A||Clinical cancer research : an official journal of the American Association for Cancer Research (10:4822)||2004|
Hyaluronic acid or TNF-alpha plus fibronectin triggers granulocyte macrophage-colony-stimulating factor mRNA stabilization in eosinophils yet engages differential intracellular pathways and mRNA binding proteins.
MA5-12394 was used in western blot to investigate the effect of tumor necrosis factor alpha and hyaluronic acid on the long-term eosinophil survival and its mechanism
|Esnault S,Malter JS||Journal of immunology (Baltimore, Md. : 1950) (171:6780)||2003|