Immunofluorescent analysis of CD44 (green) showing staining in the membrane of MDA-MB-231 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD44 monoclonal antibody (Product # MA4400) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Goat, Human, Sheep, Rabbit|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||80 - 95 kDa lymphocyte surface glycoprotein H-CAM (CD44).|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA4400 targets CD44 in flow cytometry, immunohistochemistry, immunoprecipitation, ICC/IF and neutralization applications and shows reactivity with bovine, goat, human, ovine, and rabbit samples.
The MA4400 immunogen is 80 - 95 kDa lymphocyte surface glycoprotein H-CAM (CD44). This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The metalloprotease-disintegrin ADAM8 contributes to temozolomide chemoresistance and enhanced invasiveness of human glioblastoma cells.
MA4400 was used in blocking or activating experiment to study the role of MMP and ADAM in the temozolomide-induced recurrence of glioblastoma
|Dong F,Eibach M,Bartsch JW,Dolga AM,Schlomann U,Conrad C,Schieber S,Schilling O,Biniossek ML,Culmsee C,Strik H,Koller G,Carl B,Nimsky C||Neuro-oncology (17:1474)||2015|
EGF AND TGF-alpha motogenic activities are mediated by the EGF receptor via distinct matrix-dependent mechanisms.
MA4400 was used in blocking/activating experiment to study the role of EGF receptor on EGF and TGF alpha mitogenic activities
|Ellis IR,Schor AM,Schor SL||Experimental cell research (313:732)||2007|
Fragmented hyaluronan induces transcriptional up-regulation of the multidrug resistance-1 gene in CD4+ T cells.
MA4400 was used in blocking/activating experiment to study the effect of fragmented hyaluronan on the multidrug resistance-1 gene in CD4+ T cells
|Tsujimura S,Saito K,Kohno K,Tanaka Y||The Journal of biological chemistry (281:38089)||2006|
Heterotypic cell-cell adhesion of human mast cells to fibroblasts.
MA4400 was used in blocking/activating experiment to investigate the mechanism for the interaction between HMC-1 mast cells and fibroblasts
|Trautmann A,Feuerstein B,Ernst N,Bröcker EB,Klein CE||Archives of dermatological research (289:194)||1997|
VCAM-1 and VAP-1 recruit myeloid cells that promote pulmonary metastasis in mice.
MA4400 was used in immunohistochemistry to study metastatic myeloid cell recruitment and the roles and therapeutic potential of VCAM-1 and VAP-1
|Ferjan¿i¿ ¿,Gil-Bernabé AM,Hill SA,Allen PD,Richardson P,Sparey T,Savory E,McGuffog J,Muschel RJ||Blood (121:3289)||2013|
Decreased elastin deposition and high proliferation of fibroblasts from Costello syndrome are related to functional deficiency in the 67-kD elastin-binding protein.
MA4400 was used in immunohistochemistry to assess the effect of elastin deposition in Costello syndrome
|Hinek A,Smith AC,Cutiongco EM,Callahan JW,Gripp KW,Weksberg R||American journal of human genetics (66:859)||2000|