|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||0.25 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17876 targets CD44/H-CAM in ELISA, FACS, IHC (F), IHC (P), and WB applications and shows reactivity with Mouse samples.
The MA5-17876 immunogen is mouse CD44.
CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Thymic alterations in mice deficient for the SNARE protein VAMP8/endobrevin.
MA5-17876 was used in flow cytometry to elucidate the role of VAMP8 in the thymus
|Kanwar N,Fayyazi A,Backofen B,Nitsche M,Dressel R,von Mollard GF||Cell and tissue research (334:227)||2008|
CD8+ immunoregulatory cells in the graft-versus-host reaction: CD8 T cells activate dendritic cells to secrete interleukin-12/interleukin-18 and induce T helper 1 autoantibody.
MA5-17876 was used in flow cytometry to examine interactions between alloreactive CD4 and CD8 T cells in mixed lymphocyte reactions and in the graft-versus-host reaction.
|Noble A,Leggat JA,Inderberg EM||Immunology (109:476)||2003|
On the pathogenic role of brain-sequestered alphabeta CD8+ T cells in experimental cerebral malaria.
MA5-17876 was used in flow cytometry to study the role of host immune cells in the brain of Plasmodium berghei-infected mice.
|Belnoue E,Kayibanda M,Vigario AM,Deschemin JC,van Rooijen N,Viguier M,Snounou G,Rénia L||Journal of immunology (Baltimore, Md. : 1950) (169:6369)||2002|
Integrin functions play a key role in the differentiation of thymocytes in vivo.
MA5-17876 was used in flow cytometry to investigate the role of integrin adhesion receptors in T cell development.
|Schmeissner PJ,Xie H,Smilenov LB,Shu F,Marcantonio EE||Journal of immunology (Baltimore, Md. : 1950) (167:3715)||2001|
Naïve CTLs require a single brief period of antigenic stimulation for clonal expansion and differentiation.
MA5-17876 was used in flow cytometry to determine the signaling duration requirements for priming and clonal expansion of naive cytotoxic T lymphocytes.
|van Stipdonk MJ,Lemmens EE,Schoenberger SP||Nature immunology (2:423)||2001|
Class I MHC molecules on hematopoietic cells can support intrathymic positive selection of T cell receptor transgenic T cells.
MA5-17876 was used in flow cytometry to identify cells that mediate positive selection of CD8(+) T cells.
|Zerrahn J,Volkmann A,Coles MC,Held W,Lemonnier FA,Raulet DH||Proceedings of the National Academy of Sciences of the United States of America (96:11470)||1999|