|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Myeloid cell line M1 induced with 1x10-5 M dexamethazone.|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1-0.25 µg per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Ultraviolet B suppresses immunity by inhibiting effector and memory T cells.
RM5704 was used in flow cytometry to elucidate whether UVB exposure inhibits the expansion of effector CD4+ and CD8+ T cells in skin-draining lymph nodes and reduces the number of CD4+ and IFN-gamma+ CD8+ T cells at sites of challenge
|Rana S,Byrne SN,MacDonald LJ,Chan CY,Halliday GM||The American journal of pathology (172:993)||2008|
|Not Applicable||Not Cited||
Accumulation of effector CD4 T cells during type 2 immune responses is negatively regulated by Stat6.
RM5704 was used in flow cytometry to investigate the effect of Stat6 in T cell expansion, survival, and recruitment to the lung
|King SB,Knorn AM,Ohnmacht C,Voehringer D||Journal of immunology (Baltimore, Md. : 1950) (180:754)||2008|
Active recruitment of DNA methyltransferases regulates interleukin 4 in thymocytes and T cells.
RM5704 was used in flow cytometry to investigate how T cells regulate interleukin 4 expression.
|Makar KW,Pérez-Melgosa M,Shnyreva M,Weaver WM,Fitzpatrick DR,Wilson CB||Nature immunology (4:1183)||2003|
Involvement of p38 mitogen-activated protein kinase in different stages of thymocyte development.
RM5704 was used in flow cytometry to suggest that p38 is involved in positive selection of T cells.
|Hsu SC,Wu CC,Han J,Lai MZ||Blood (101:970)||2003|
Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection.
RM5704 was used in flow cytometry to test if T-cell-specific expression of p300 alters GR-TCR cross talk between thymocytes.
|Yu CT,Feng MH,Shih HM,Lai MZ||Molecular and cellular biology (22:4556)||2002|
Commitment of common T/Natural killer (NK) progenitors to unipotent T and NK progenitors in the murine fetal thymus revealed by a single progenitor assay.
RM5704 was used in flow cytometry to describe a clonal system that supports the development of T and natural killer cells evenly.
|Ikawa T,Kawamoto H,Fujimoto S,Katsura Y||The Journal of experimental medicine (190:1617)||1999|
Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4.
RM5704 was used in flow cytometry to identify the initial source of IL-4 in early immune responses.
|Liu L,Rich BE,Inobe J,Chen W,Weiner HL||International immunology (10:1017)||1998|