|Tested species reactivity||Dog, Horse, Cat, Human, Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/ml|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Can CD44 Be a Mediator of Cell Destruction? The Challenge of Type 1 Diabetes.
MA1-10225 was used in immunohistochemistry to investigate how CD44 modulation induced resistance to type 1 diabetes in NOD mice
|Assayag-Asherie N,Sever D,Bogdani M,Johnson P,Weiss T,Ginzberg A,Perles S,Weiss L,Sebban LE,Turley EA,Okon E,Raz I,Naor D||PloS one (10:null)||2015|
Continuous activation of autoreactive CD4+ CD25+ regulatory T cells in the steady state.
MA1-10225 was used in flow cytometry to report two Treg subsets with distinct phenotypes and homeostasis in normal unmanipulated mice.
|Fisson S,Darrasse-Jèze G,Litvinova E,Septier F,Klatzmann D,Liblau R,Salomon BL||The Journal of experimental medicine (198:737)||2003|
CD8+ tumor-infiltrating lymphocytes are primed for Fas-mediated activation-induced cell death but are not apoptotic in situ.
MA1-10225 was used in flow cytometry to characterize murine tumor-infiltrating lymphocytes.
|Radoja S,Saio M,Frey AB||Journal of immunology (Baltimore, Md. : 1950) (166:6074)||2001|
|Not Applicable||Not Cited||
Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.
MA1-10225 was used in flow cytometry to study CD44 and hyaluronic acid interactions
|Lesley J,He Q,Miyake K,Hamann A,Hyman R,Kincade PW||The Journal of experimental medicine (175:257)||1992|