Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 400x magnification with DAPI counterstain. Primary antibody used was a CD44/H-CAM monoclonal antibody (clone KM81) at a 1:100 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-rat at a 1:500 dilution and Normal Goat Serum (blocking)
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Bone Marrow Derived Stromal Cells (clone BMS2).|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
MA1-70080 detects CD44 in mouse samples.
MA1-70080 has been successfully used in flow cytometry procedures.
The MA1-70080 immunogen is bone marrow derived stromal cells (clone BMS2).
The molecular weight of CD44 is 95 kDa.
Store at 4°C short term; store at -20°C long term, avoiding freeze-thaw cycles.
CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Molecular isoforms of murine CD44 and evidence that the membrane proximal domain is not critical for hyaluronate recognition.
MA1-70080 was used in flow cytometry to determine the importance of the membrane proximal region of murine CD44 for recognition of hyaluronate
|He Q,Lesley J,Hyman R,Ishihara K,Kincade PW||The Journal of cell biology (119:1711)||1992|